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VACCIJ^ATION {mcdnia)— 
A. History. 
B. Methods of production ; 
Vaccine lymph. 
Vaccine pulp — 
Dry points. 
Glycerinated pulp. 
C. Technique of vaccination. 
D. Immunity produced. 
E. Bacteriologic and microscopic study of vaccine matter. 
F. Bodies in epithelial cells — cornea of rahbits. 
Examination of vaccine — 
G. Note the name of maker, kind of virus, etc. 
H. Count the number of colonies upon : 
1. Three glycerinated tubes upon agar. 
2. Three glycerinated tubes or points upon gelatin. 
3. Three dry points upon agar. 
4. Three dry points upon gelatin. 
I. Study the character of the organisms found upon media and animals. 
J. Plant the entire contents of 6 tubes in bouillon, grow anaerobically and study the 
characters of the growth upon media and by inoculation in animals. 
K. Study the dry points as in J. 
L. Inoculate the contents of 12 tubes and 12 points directly into mice, rats, and 
guinea pigs. 
M. Keep full notes of all results. 
N. Visit to the vaccine farm for practical study of manufacture of vaccine com- 
mercially. 
