11 
POINTS TO bp: observed IX DESCRIBING AN ORGANISM— 
1. Its source. 
2. Ilaiifjing drop. P'orin, size, ^roujiin^ an<l ihotility. 
II. Spores. 
4. Staining j)eculiarities. 
5. P'lagella. 
6. Appearance of colonies on gelatin, agar, blood-serum, |)<ttato, etc. 
7. Relation to oxygen. 
S. Growth on gelatin. 
9. Growth on agar. 
10. Growth on blood-serum. 
11. Growth on potato. 
12. Growth on fluid media: 
a. Bouillon — 
1. Acid. 
2. Neutral. 
8. Alkaline. 
I). Dunham’s solution — indol. 
18. Milk and litmus milk. 
14. Reaction of media best suited to growth. 
15. Changes in reaction of media. 
1(). Gas production: 
a. Glucose bouillon. 
h. Lactose bouillon. 
r. Saccharo.se bouillon. 
d. Mannite bouillon. 
e. Gluco.se agar. 
/. Glucose gelatin. 
17. Temperature best suited to development. 
IS. Pathogenicity. 
19. Reaction to sera of immune animals, antilxxlies, precipitins and agglutinins. 
20. Toxins. 
