15 
I 
>rAKIXG l‘LATKS, I-ITC. 
1. Koch’s method — glass i)lates. 
, 2. Esmarch’s roll tul)es. 
' 3. (Slants of agar and gelatin in test tuhes, 
4. Petri dishes — 
j (Streaking on surface with platinum wire. 
Daubing with swabs on surface. 
1 Mixing while tiuid. 
X)bsekvk (Growth and I ulti plication of I^vctekia in Cell Slide — Aoar 
Method: 
1. Division of bacilli, 
j 2. Division of cocci. 
' 3. (Sporulation. 
1 Study of Pkownian ]\Iove.ment with — 
India ink and other substances in hanging drop. 
j, C ounting Colonies, i. e., number of liaeteria. 
f 1. Petri dishes — 
bsTAiNiNG Puke Cultures — 
1. l>y plate method on solid media. 
I 2. Dy dilution in liijuid media. 
3. Dy temperature. 
' 4. Inoculation into animals. 
I 5. Negative plates. 
Ifi , 6. (Special methods. 
Aim to have not over 200 colonies on the itlale. 
Count with a lens X o D. 
r 
'Wolllugel’s counting ai>paratus. 
II. W. Jeffer’s plate. 
V 2. Esmarch’s roll tubes. 
3. Dy dilution in licpiid media. 
