20 
PREPARATION AND ^lOUNTING OF HISTOLOGIC SPECIMEN 
1. Fixation: 
a. Acetic-bichlorid of mercury. 
h. Muller’s fluid. 
c. Chrom-acetic acid. 
d. Flemming’s solution. 
e. Formaldehyd. 
/. Absolute alcohol. 
2. Dehydration: 
rt. Alcohol 70 per cent (iodin alcohol if HgCL). 
h. Alcohol 85 per cent. 
c. Alcohol 95 per cent. 
d. Absolute alcohol, twice. 
3. Clearing: 
a. Xylol. 
h. Chloroform. 
c. Turpentine. 
d. Oil cloves. 
e. Oil bergamot. 
/. Oil origanum. 
I. Infiltration and imbedding: 
a. Paraflin. 
h. Celloidin. 
5. Sectioning: 
a. Single sections. 
h. Serial sections. 
6. Attaching section to slide: 
a. Alcohol 80 per cent. 
h. Water. 
c. Myer’s albumen. 
d. Gum Arabic. 
e. Collodium and clove oil. 
7. Removal of imbedding material: 
a. Xylol to remove paraffin. 
b. Alcohol and water. 
8. Staining: 
a. Nuclear staining. 
5. Protoplasmic. 
c. Regressive. 
d. Gram’s. 
e. Bacterial. 
/. Special. 
9. Dehydration in absolute alcohol, anilin oil. 
10. Clearing: 
a. Xylol. 
6. Turpentine. 
c. Oil origanum. 
d. Oil bergamot. 
e. Oil cloves. 
/. Chloroform. 
11. Mounting in xylol balsam, or gum thus. 
