28 
METHOD OF INFAKINO FROZEN SECTIONS. (Wri^^ht.) 
1. Place tissue not over 5 imn. thick in 10 per cent formalin for two or more hours. 
2. "Wash in water. 
3. Freeze with CO 2 or ether. 
4. Cut frozen sections. 
5. Float sections in normal salt .s<jlution and then on slide. 
0. C’ovcr section with cigarette }>aper, then with blotter moistened with 95 per 
cent alcohol. Press. Remove i>apers. 
7. Cover with absolute alcohol thirty seconds. Drain. 
8. Flow over the section and adjacent parts of tlie slide a thin solution of celloidin 
in ecpial parts of absolute alcohol and ether. Drain off immediately. The 
celloidin should form a coating so thin as to be invisible. 
9. Flood the slide with 95 per cent alcohol and then immerse in water. This 
hardens the film. 
10. Stain. 
11. Dehydrate. 
12. Clear with oil origanum. 
18. Mount in Canada balsam. 
