52 
BLOjI). 
Techni*:itie of: 
1. Collecting: for micfoso^pic examiiiiition. 
Collecting bloo*l for bacteriol‘Jgic exaniination — 
«. Small amonnt. 
b. Liirge qnantiti*:^. 
Of man. 
C>f other manimaL*. 
Of binis- 
C>f reptiles. 
3. 01>serTe circnlation of blo*>l Tm.*_ler imcr*:)6«.-oj>e. 
Mesentery of frog. 
-T. Estimate alkalinity. 
5. Estimate specific gravity of bloo*i witli niivmre of cMorofoim and l»enz*>i 
6. Examine- fresh Moo*i in hanging *ir*>p. 
- 7. Examine fresh l>Io*:xl Ijetween slide and cover slip. 
.s. Study of blooti films: 
a. Metho»i ■«rith cover glass. 
b. Method vrith special red. 
3Iethcd with cigarette paj;>er. 
9. EixatiorL of bloot;l films: 
ef. Heat, 150' C. for ab*)nt five minntes. 
K Alcohol and ether. 
c. Formalin. 
d. Formaielehyd. 
r. Acetic acid bichlorid. 
/. IV ocd alcohol. 
10. Staining: 
«. Ehrlieh-Biondi-tri-acid stain. 
b. Jenner s stain. 
c. Bc»manowsky's stain, 
d. Goldhorn’s fi^nid. 
e. Eosin and methylene bine, 
f. Thionin and eosin. 
g. Wright’s. 
h. Hematoxylin and eosin. 
11. Counting re»l corpuscles. 
Thoma-Zeiss hemocytometer. 
12. Estimating the number of leucocytes: 
a. With the Thoma-Zeiss hemocytometer. 
b. Comparative count of — 
«. Small lymphocytes. 
b. Large lymphocytes and transitional forms. 
c. Polymorphonuclear neutrophiles. 
d. Ecdnophiles. 
€. Mast cells. 
13. Estimating the amount of hemoglobin: 
With— 
a. Hare A hemoglobinometer. 
b. Oliver s hemo'globinomcter. 
t. Gower s hemoglobinometer. 
