65 
' VACCIXATIOX {vaccinia ) — 
1 A. History. 
B. Methods of production: 
Vaccine lymph. 
Vaccine pulp — 
I Dry points. 
Glycerinated pulp. 
C. Technique of vaccination. 
D. Immunity produced. 
E. Bacteriologic and microscopic study of vaccine matter. 
F. Bodies in epithelial cells — cornea of rabbits. 
Xote the name of maker, license number, kind of virus, <late of return, etc. 
a. Count the nund^er of colonies per point. 
Immerse the point in 1 cc. physiological salt solution 15 minutes to soften the 
vaccine matter. 
Scrape well and agitate to break up clumps, etc. 
Plate the emulsion thus obtained in three Petri dishes as follows: 
5 drops in first. 
10 drops in second. 
Remainder in third. 
Then plant in melted agar, incubate at 87° C. for three days, then room 
temperature for two days. 
Count number and determine character of colonies. 
h. Count number of colonies per capillary tube. 
Wash contents of tube into 1 cc. sterile water. 
Agitate to break up clumps. 
Plate in agar as above. 
c. Plant the entire contents of 10 tubes or 10 points in 100 cc. of freshly prepared 
and recently boiled glucose bouillon. 
Incubate at 37° C. 
At the end of 48 hours inoculate upper growth into peritoneal cavity of a 
rabbit, using 1 cc. per 1,000 gm. of rabbit. 
If rabbit sickens or dies determine cause of the infection. 
At the end of seven days filter the bouillon growth through porcelain and 
inoculate 0.2 cc. of the filtrate subcutaneously into a mouse for tetanus 
toxin. 
27923— Xo. 8—04 5 
The examination of vaccine under the law of Jubj i, 1902. 
