0 
morniDg with signs of tetanus, such as stiff tail and retraction to right 
side. Smears made from the site of inoculation showed the ]>resence 
of a thin rod with a round end-spore. Decreasing doses were put into 
white mice on successive days until finally it wjis found that .000022 
c. c. would kill a mouse with typical symptoms of tetanus in four days, 
with an incubation period of about thii ty-six hours 
SUMMARY AND CONCLUSIONS. 
Seven samples of gelatin examined; one showed tetanus spores. 
Two samples showed an oval end -spore rod, whose identity was not 
proved, but in stained specimens it would be hard to distinguish from 
tetanus, if indeed not tetanus with diminished virulence. 
In tetanus investigations it is important to use freshly made bouillon, 
as the organism is apt not to germinate in bouillon over ten days old. 
The thermal death point of the organism isolated wiis found to be 
between twenty and thirty seconds at 100° C. 
It is important, therefore, that gelatin to be used for injections should 
be boiled at least ten minutes on account of the variability of the ther- 
mal death point in different species of tetanus. Whether this amount 
heating impairs in any way the hemostatic power of gelatin has not 
been settled, but in case it does it is believed that the danger from 
tetanus more than overbalances its therapeutic value. 
It is suggested that when, as in hospitals, there is likelihood of gela- 
tin injections being used for hemostatic purposes the gelatin solution 
be sterilized by the fractional method on three successive days and kept 
ready for use in sterile containers. 
