12 
The following tables give the results of our counts, classified accord- 
ing to the manufacturer. They are given in three senes: First, the 
result of our work during the winter of 1901-2; second, the result of our 
work during the summer (April and May); and, third, in November 
and December, 1902. We were surprised to find such a large number 
of colonies, especially from the glycerinated virus, and therefore 
repeated our work veiw carefully. The counts were made by Dr. 
Grubbs, Dr. Parker, Dr. Francis, and Dr. Anderson. 
NUMBER OF BACTERIA IN VACCINE VIRUS BOUGHT ON THE OPEN 
MARKET, WITH A SPECIAL STUDY OF THE PYOGENIC COCCI. 
The number of bacteria found in vaccine virus is of course not so 
important a factor as the kind of organisms. A few tetanus spores or 
virulent pus cocci would be of far more serious moment than myriads 
of the common saprophytes. However, the number of organisms 
found to contaminate vaccine virus is a fair index of its purity, as 
well as of the care taken in its production. The number of bacteria 
found in glycerinated virus is also an indication of its “ripeness.” 
We counted 190 dry points and 244 glycerinated tubes. The dry 
points averaged 4,354 bacteria per point and the tubes 1,742 per tube. 
Some points contained as great a number as 44,000 foreign organ- 
isms. The highest count found in the capillary tubes containing 
glycerinated virus was 30,080. A few (very few) points and tubes 
were sterile, i. e. , gave no bacterial growth either on agar plates or in 
bouillon. 
