DRY POINTS AND GLYCERINATED VIRUS COOTARED FROM A BACTERIO- 
LOGICAL STANDPOINT. 
The question is often asked. Is glycerinated virus superior to dry 
points? 
As the object of glycerinating the virus is to eliminate the foreign 
bacteria, the answer to this question resolves itself almost entirely 
into a comparative study of the number and kind of organisms found 
to contaminate this kind of vaccine. 
The result of our studies on this point may be summarized as 
follows : 
Series. 
A Average number of 
organisms. 
Dry points. 
Glycerinated 
rims. 
Series I, Avinter, 1901-2 
4, 809 
4, 698 
1,058 
29 
Series II, spring, 1902 
Series III, Avinter. 1902-3 
3, 4.58 
4, 895 
It is plain that last winter (Series I) the glycerinated virus contained 
a number of contaminating bacteria equal in amount to the dry points. 
This winter (Series III) shows a great improvement in the glycerinated 
'vdrus and demonstrates conclusive!}’ that this form of vaccine may be, 
and should be freer from impurities than the dry points. 
Of course the greater freedom from bacterial impurities of the 
glycerinated virus to the dry points refers only to those dry points 
made with fresh *Tymph." There can be little choice between the dry 
points made with glycerinated pulp and the capillary tubes containing 
the same material. 
The pyogenic cocci and other bacteria pathogenic for laboratory 
animals were found both in the glvcerinated virus and on the drv 
points. 
EXPERIMENTAL TETANUS IN VACCINE VIRUS. 
Can tetanus be found in the vaccine virus bought in open market ? 
'Many tubes containing glycerinated virus and many points of the 
dry vaccine were examined bacteriologically for tetanus, but we were 
unable to discover this organism. 
This part of the work was done in several ways. Susceptible ani- 
mals were inoculated and vaccinated with the contents of many tubes 
38 
