40 
quantity of pure bouillon culture indicated was inoculated into the 
rio’ht side of a mouse. 
o 
( 
' Mouse. 
1 
1 
Quantity ol , 
tetanus 
culture 
injected on 
March 9. 
! 
1 
c. c. 
0. 000065 
2 
. 0000-19 
3 
. 000039 
1 4 
. 000032 1 
5 
. 000028 1 
6 
. 000024 
" 
. 000021 
On March 10 the three mice which had received the largest doses 
showed typical s^^mptoms of tetanus, while the four mice which had 
received the smallest doses showed no symptoms. 
On March 11 the mouse which received the largest dose was dead. 
The second, third, fourth, fifth, and sixth showed typical symptoms 
of tetanus, while the seventh, which received the smallest dose, gave 
no symptoms whatever of tetanus. 
On March 12 mice 2 and 3 were dead; 4, 5, 6, and T show typical 
tetanus. 
On March 13 mice 4, 5, and 6 were dead; T shows t}"pical tetanus. 
On March 14 mouse T died. 
Table shouing virulence of the tetanus culture used in these experiments. 
Mouse. 
March 9. 
March 10. 
March 11. 
March 12. 
March 13. 
March 14. 
1 
c. c, 
0. 000065 
Typical 
tetanus. 
Dead. 
2 
. 000049 
do 
Typical 
tetanus. 
Dead. 
3 
. 000039 
do 
do 
Do. 
4 
. 000032 
Negative . 
do 
Typical 
tetanus. 
Dead. 
5 
. 000028 
do 
do 
do 
Do. 
6 
. 000024 
do 
do 
do 
Do. 
7 
. 000021 
do 
Negative . 
do 
Typical 
tetanus. 
Dead. 
1 
From these tests we considered 0.000021 c. c. to constitute about the 
minimum fatal dose of our culture for an average-sized white mouse. 
VIABILITY OF A PUEE CULTURE OF TETANUS IN GLYCERINATED VACCINE VIRUS. 
The vaccine pulp was taken from the heifer on February 15 and 
subjected to the action of glj^cerin by the vaccine manufacturer for 
twenty-five da\^s. It was then, on March 12, obtained b}" us and con- 
taminated with our pure culture of tetanus in the following manner: 
