t 9 
will be noted that the virulence beg’an to diminish noticeably at the i 
fourth month and failed to produce tetanus after the lapse of live | 
months. 
Capillary tubes containing 0.00011 c. c. of tetanus culture added to glycerinated virus, 
March 12, and tested at inten'als as shown. 
1 Date 
prepared. 
! 
Inocu- 
lated 
into 
mice. 
Num- 
ber 
of 
days. 
Symptoms. 
1st 
day. 
2d 3d 
day. day. 
4tb 5th 6th 7th 8th 
da}’, day. day. day. day. 
9th 
day. 
Result. 
10th 
day. 
, Mar. 12 . . 
June 17 
97 
N 
P P 
Positive: death. 
' Do... 
Aug. 28 
169 
N 
N N 
X 
Negative. 
Do... 
Sept. 3 
1 75 
X 
N X 
X 
Do. 
Do... 
Sept. 9 
ISl 
N 
X X 
X 
1 
Do. 
The series contained approximately live times the minimal fatal dose 
of tetanus. It was first tested after the lapse of three months, when 
it was still virulent: but after seven months it had entirely lost its 
power of producing the disease when inoculated directh^ into mice, 
Evidently the tetanus spores did not germinate or produce toxin. 
Ca'pillary tubes containing 0.00022 c. c. of tetanus culture added to glycerinated virus, 
March 12, and tested at intervals as shown. 
Date 
pre- 
pared. 
Inocu- Xum- 
lated her 
into of 
mice. days. 
Symptoms. 
Result. 
1st 
day. 
2d 
day. 
3d 
day. 
4th 
day. 
’ 
5th 
day. 
6th 
day. 
7 th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
Mar. 12 
Sept. 1 173 
X 
X 
P 
P 
P 
P 
P 
P 
P 
P 
Positive; recovered. \ 
Do... 
Oct. 1 203 
Is 
P 
P 
P 
P 
P 
Do. 
Do.. 
Xov. 15 248 
X 
P 
P 
P 
P 
P 
P 
Do. 
Do... 
Jan. 1 295 
X 
Negative. 
This series was contaminated with ten times the' minimal fatal dose, j 
I n this large Cjuantity the spores maintained their toxicity a very long 
time — two hundred and forty-eight days — although they failed to pro- ;; 
duce the disease after ten months. 
Cajjillary tubes containing O.OOO 4 O c. c. of tetanus culture added to glycerinated virus, 
March 12, and tested at intervals as shown. 
Date 
pre- 
pared. 
1 
1 
Inocu- Xum- 
latea her 
into of 
mice. days. 
Symptoms. 
Result. 
1st 
day. 
2d 
day. 
3d 
day. 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
Mar. 12 
Sept. 1 173 
X 
P 
P 
P 
P 
P 
P 
P 
P 
T 
Positive; death. 
Do.., 
Oct. 1 203 
X 
P 
P 
P 
P 
P 
P 
P 
P 
p 
Positive; recovered. 
Do... 
Xov. 15 248 
X 
X 
P 
P 
P 
P 
P 
P 
P 
p 
Do. 
Do... 
Jan. 1 295 
X 
Negative. 
