43 
Each capillaiy tube in this series contained about twent}^ times the 
minimal fatal dose of tetanus, which lost its virulence after ten 
months. 
Capillary tubes containing 0.00074 c. c. of tetanus culture added to glycerinated virus, 
March 12, and tested at intervals as shovn. 
Date 
pre- 
pared. 
Inocu- 
lated 
into 
mice. 
Num- 
ber of 
days. 
Symptom.s. 
Result. 
1st 
day. 
2d 
day. 
3d 
day. 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 10th 
day. day. 
:Mar. 12 
Sept. 1 
173 
P 
P 
t 
Positive: death. 
Do... 
Oct. 1 
203 
P 
P 
P 
P 
P 
P 
P 
P 
P t 
Do. 
Do... 
Xov. 15 
248 
P 
P 
J. 
Do. 
Do... 
Jan. 1 
295 
P. 
P 
Do. 
Do... 
Feb. 1 
326 
N 
P 
Do. 
Do... 
Mar. 2 
355 
X 
P 
i 
Do. 
It will be seen that this large amount of tetanus (about forty times 
the minimal fatal dose) remained alive and virulent in glycerinated 
virus one year. 
Capillary tubes containing 0.013 c. c. of tetanus culture added to glycerinated virus, March 
12, and tested at intervals as shown. 
Date 
pre- 
pared. 
Inocu- 
lated 
into 
mice. 
Num- 
ber of 
days.. 
Mar. 12 
Sept. 1 
173' 
Do... 
Oct. 1 
^03 
Do... 
Nov. 15 
248 
Do... 
Jan. 1 
295 
Do... 
Feb. 1 
326 
Do... 
Mar. 2 
355 
Symptoms. 
1st 
day. 
2d 
day. 
3d 
day. 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
P 
P 
P 
P 
P 
N 
t 
t 
t 
t 
t 
P 
t 
Result. 
Positive; death. 
Do. 
Do. 
Do. 
Do. 
Do. 
This large amount (about 500 X MLD) has also remained alive and 
virulent for mice one year. The material is not yet exhausted, and 
will be tested from time to time. 
VIABILITY OF PURE CULTURE OF TETAXUS OX DRY POIXTS. 
The serum which exuded from the site of the inoculation after the 
superficial Wers of the variolous eruption was removed was obtained 
from a calf on March 12, 1902. This serum, commonly called 
lymph,” when dipped or brushed upon the ivory points and allowed 
to dry, formerly constituted the “dry points” of the manufacturers. 
At present some diy points are made with glycerinated virus alone or 
with glycerinated pulp mixed with blood serum and in other wa}"s. 
We, however, preferred to use the “lymph” or serum, as it seemed to 
