45 
directly into mice. But when grown anaerobically, as above, the 
spores multiplied and regained their pathogenic power. 
Dry points containing vaccine serum lymph”) ivere contaminated ivith 0.0004 c. c. of 
, tetanus cidture and kept cd 22° C. in the dark. From time to time they were tested, with 
the following result: 
Date 
pre- 
pared. 
Inocu- 
lated 
into’ 
mice. 
3 5^0 S' 
Symptoms. 
Result. 
1st. 
day. 
2d 
day. 
3d 
day. 
4tli 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
‘ 
10th 
day. 
■ 
Mar. 12 
Do... 
Do... 
May 28 
June 17 
June 27 
77 
107 
117 
P 
X 
N 
P 
P 
P 
P 
P 
P 
P 
P 
P 
Positiye; recoyered. 
Xegatiye. 
Do.. 
This series of diy points, contaminated with about fort}- times the 
minimal dose of tetanus, remained virulent over sevent 3 ^-seven davs, 
but failed to produce sjunptoms after one hundred and seven da 3 ’s. 
The spores, however, were not dead; for, planted in bouillon and' 
grown in a Novy jar for seven davs, the}' gave a rich culture, which 
produced typical symptoms of tetanus in a mouse. 
Dry points containing vaccine serum lymph”) ivere contamincdecl with 0.001 c. c. of 
tetanus cidture. From time to time they were tested, with the following result: 
Date pre- 
pared. 
• 
Inocu- 
lated 
into 
mice. 
X’um- 
ber 
of 
days. 
Symptoms. 
Result. 
1st. 
day. 
2d 
day. 
3d 
day. 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
TMar 12 
May “18 
P 
t 
Positiye; death. 
Do 
■Tirne 27 
107 
P 
' P 
t 
Do. 
Do 
.Aue". 1 
142 
9 
P 
P 
P 
P 
t 
Do. 
Do 
Aug. 28 
169 
X 
P 
t 
Do. 
Do 
Sept. .30 
202 
hr 
P 
Positiye. 
’ Do 
Xoy. 15 
248 
p 
P 
P 
P 
P 
P 
P 
P 
P 
t 
Positiye; death. 
Do 
Jan. 1 
295 
X 
X 
P 
P 
P 
P 
P 
P 
t 
Do. 
Do 
Feh. 1 
336 
X 
Xegatiye. 
The large amount of tetanus in this series of points remained viru- 
lent, when direct!}' inoculated into mice, for a period of two hundred 
and ninety-five days. This shows well the long life of dried tetanus 
spores ^hen kept in a cool place, protected from the light — that is« 
under the conditions under which vaccine virus is usually conserved. 
VIABILITY OF TETANUS IN MIXED CULTURE IN GLYCERINATED VIRUS. 
In the following experiments we used very small quantities of a 
culture of tetanus mixed Avith other organisms, viz, a coccus and a 
saprophytic motile rod with a central spore. This mixture was planted 
into ordinary bouillon April 1 and incubated at 37^ C. in a NoA'y jar 
