5 
Table II. 
Bouillon with the addition of ferrous sulphate in various percentages contaminated 
by the addition of a small quantity of garden earth. Kept in the incubator. 
Proportion of fer- 
rous sulphate. 
First day. 
Second day.- 
Third day. 
Fifth day. 
Fourteenth 
day. 
1 : 200 
Yerv foul odor 
No odor. 
1 
1 : 150 
Foul odor 
1 : 100 
do 
1 :50 
No odor 
Foul odor 
1:35 
do 
do 
1:30 
do 
do 
1:28 
do 
No odor 
Foul odor 
1 : 25 
do 
do 
No odor 
Foul odor 
do 
1 : 23 
do 
do 
do 
1 : 20 
do 
do 
do No odor 
GERWICIDAL ACTION OF FERROUS SULPHATE. 
Forty-eight hour bouillon cultures of B. typhosus, B. coli communis, B. pyocyaneus, 
vibrio cholera Asiat., strong aqueous suspensions from forty-eight hour cultures on 
agar of B. anthracis, B. subtilis, Staphyl. pyog. aureus and albus, and a strong sus- 
liension of a forty-eight hour growth of B. diphtheria on blood serum were used to 
contaminate slips of filter paper, silk threads, and glass cover slips. After being con- 
taminated by the culture the paper slips, silk thread and cover slips were placed in 
the incubator until they were almost dry (in the case of anthrax and subtilis they 
were allowed to dry completely), then the disinfecting agent was applied for definite 
periods, its action stopped by careful washing in sterile water, the slips and threads 
planted in bouillon and placed in the incubator for forty-eight hours. 
Table III. — Infected material, filter paper. 
Result after application of sat. sol. of ferrous sulphate. 
ui^uuisiii eiiipiuN eu. 
o' minutes. 
10 minutes. 
20 minutes. 
30 minutes. 
45 minutes. 
60 minutes. 
B. anthracis 
"T 
+ 
+ 
-K 
B. subtilis 
-f 
-j- 
+ 
H- 
4_ 
+ 
B. tvphosus 
_U 
-f- 
+ 
— 
B. coli communis 
— 
\ 
“T 
-f 
+ 
B. pvocvaneus ^ . 
-f 
■ + 
"V 
4 
Vib. cholera Asiat 
_L 
4- 
4" 
B. diphtheria 
-r 
-1- 
-h 
4 - 
-f 
-f 
Staphyl. pvog. aureus 
-4- 
-f 
+ 
4" 
Staph Vl. epider. albus 
-1- 
"T 
4“ 
4 
-i- 
+ 
Table IV . — Infected material, silk threads. 
Organism employed. 
Result after application of saturated solution of ferrous sulphate. 
5 minutes. 
10 minutes. 
20 minutes. 
30 minutes. 
45 minutes. 
60 minutes. 
B. anthracis 
U- 
4- 
4“ 
4- 
-+ 
4- 
B. subtilis 
4“ 
4- 
4- 
4“ 
+ 
4_ 
B. tvphosus 
4- 
4- 
4- 
4- 
4 - 
B. coli communis 
4- 
4- 
A- 
4" 
4“ 
B. pvocvaneus 
4- 
4- 
-f 
4- 
+ 
4- 
B. diphtherise 
4" 
4- 
4- 
4- 
4- 
+ 
Vibrio cholera Asiat 
4- 
4- 
+ 
4- 
4- 
4* 
Staphyl. pyogenes aureus 
+ 
-4 
4- 
4- 
_1_ 
4- 
Staphvl. epider, albus 
-h 
4- 
■■ + 
4- 
4- 
