6 
Table V. — Infected material, glass cover slips. 
Result after application of saturated solution of ferrous sulphate. 
Organism employed. 
5 minutes. 
10 minutes. 
20 minutes. 
30 minutes. 
45 minutes. 
60 minutes. 
B. anthracis 
I 
“T 
+ 
+ 
B. subtilis 
-i- 
+ 
~V 
B. tvphosus 
— 
4. 
T 
— 
B. coli communis 
+ 
-y 
_L- 
_L 
B. pvocvaneus 
-t- 
-\r 
-r 
B. diphtherise 
_u 
4- 
+ 
4- 
Vibrio cholera Asiat 
-r 
_I_ 
— 
Staphyl. pyogenes aureus 
-f- 
+ 
4- 
“T 
Staphyl. epider. alhus 
4- 
Some of the pathogenic organisms tested (V. cholerae and B. typho- 
sus) did not grow after one hour exposure to the iron solution; but in 
the following experiments upon feces it will be seen that Y. cholera 
was recovered from feces after forty-eight hours exposure to a 
saturated solution. In the experiments upon feces the saturated 
solution of ferrous sulphate constituted twice the bulk of the feces 
and was intimately mixed with it. and all lumps broken up to make as 
nearly as possible a homogeneous mixture. 
Table VI. 
Twenty-five grams of feces thoroughly mixed with twice its bulk (50 c. c.) of a 
saturated solution of ferrous sulphate. At definite intervals a platinum wire loopful 
was planted in bouillon and placed in the incubator twenty-four hours, with the 
results given below. 
5 minutes 
10 minutes 
20 minutes 
30 minutes 
40 minutes 
50 minutes 
1 hour 
2 hours . . 
18 hours . . 
24 hours . . 
48 hours . . . 
72 hours . . 
Time of exposure to iron solution. 
Result. 
Good growth. 
Do. 
Do. 
Do. . 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Table VII. 
Twenty-five grams feces thinned by the addition of 10 c. c. bouillon culture of 
pyocyaneus and thoroughly mixed with twice its bulk of a saturated solution of 
ferrous sulphate. A platinum wire loopful of the mixture was planted at definite 
intervals in bouillon and placed in the incubator for twenty-four hours, with the 
result indicated below. 
Time of exposure to iron solution. Result. 
1 hour . 
2 hours 
3 hours 
4 hours 
5 hours 
6 hours 
24 hours 
48 hours 
72 hours 
Good growth. B. pvocvaneus recovered. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
