12 
ture of Staphylococcus pyogenes aureus taken from the surface of an agar slant free of 
foreign organic matter. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c. ) of the test fluids and also 
one of the slips of filter paper was planted in bouillon. 
■ [4- means growth; — means no growth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
Per cent 
glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.: 
7 days 
+ 
-!- 
4- 
— 
— 
r 
“T 
— 
— 
4- 
— 
13 days 
20 days 
27 days 
+ 
34 days 
4- 
41 days 
I 
.... 
48 days 
_L 

55 days 
Room, 22° to 24° C.: 
7 days 
4- 
4- 
1 
4- 
4- 
— 
4- 
4- 
4- 
4- 
— 
13 days 
-r 
4- 
+ 
+ 
20 days 
4- 
27 days 
-i- 
34 days 
) 
4_ 
41 days 
Ice chest, 10° to 12° C.: 
.... 
7 days 
+ 
4- 
+ 
-1- 
4- 
+ 
“F 
4- 
+ 
+ 
+ 
4- 
13 days 
+ 
+ 
-1- 
4- 
4- 
4- 
-f 
"T 
4- 
4- 
4- 
— 
20 days 
+ 
+ 
4- 
4- 
4- 
— 
— 
4- 
4- 
4- ■ 
4_ 
— 
27 days 
+ 
4- 
4- 
4- 


-f 
4 
34 days 
4- 
4- 
4- 
4- 
41 days 
_1_ 
4_ 
. 
4- 
4- 
48 days 
4- 
.... 
55 days 
4- 
62 days 
4- 
69 days 
76 days 
O- 
83 days 
4- 
90 days 
4- 
i 
97 days 
; 
Ill days 
1 
1 
STAPHYLOCOCCUS EPIDEEMIS ALBUS. 
The various percentages of glycerin were made with sterile distilled water and 
distributed into test tubes. Each tube was abundantly inoculated with a young 
culture of Staphylococcus epidermis cdbus, taken from the surface of an agar slant, free 
of foreign organic matter. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile shps of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c. ) of the test fluids and also one 
of the slips of filter paper were planted in bouillon. 
