14 
[+ means growth; — means no groAvth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
Per cent 
glycerin. 
10 
20 
30 
40 
50 
60 
1 
70 
80 
90 
lOO 
Room, 22° to 24° C. — Continued. 
fiRdavs 
4- 
70 davs 
T 
77 days 
84 davs 
91 davs 
98 davs. 
112 da vs 
1 8.8 da vs 
Ice chest, 10° to 12° C.: 
7 davs 
+ 
-h 
-h 
4- 
“T 
T' 
4- 
4- 
4- 
-r 
4- 
14 davs 
4- 
J- 
1 
4- 
+ 
4- 
4- 
4- 
4- 
4- 
4- 
4- 
19 days 
+ 
+ 
+ 
4- 
+ 
+ 
4- 
4- 
4- 
4- 
4- 
4- 
29 days 
+ 
-1- 
4- 
4- 
+ 
_L 
4- 
4- 
+ 
+ 
— 
— 
35 days 
+ 
+ 
4- 
4- 
+ 
4- 
4- 
4- 
4- 
42 d a ys . 
+ 
4- 
4- 
+ 
4- 
49 days 
4- 
4- 
4- 
56 days 
-f- 
4- 
.... 
63 davs - 
+ 
4- 
(a) 
70 days. 
-1- 
_ 1 _ 
... 

77 days 
-1- 
4- 
84 days 
4- 
4- 
■ 
91 davs 

+ 
4- 
98 da vs - - . 
+ 
4- 
112 days 
4- 
133 days 
4- 
1.54 davs. _ V- 
1 
(&) 
! 
a Slow. b Material exhausted. 
DIPHTHEEIA. 
The various percentages of glycerin were made with sterile distilled water and dis- 
tributed into test tubes. Each tube was abundantly inoculated with a young culture 
of dipththeria, taken from the surface of an agar slant, free of foreign organic matter. 
For comparison, the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. * 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c. ) of the test fluids and also one 
of the slips of filter paper were planted in bouillon. 
[+ means growth; — means no gro'wth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
Per cent glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.: 
7 days 
14 da5's 
— 
4- 
— 
19 days 
Room, 22° to 24° C.: 
7 
— 
7 
4- 
4- 
-h 
- 
4- 
14 days 
19 days 
29 days 
Ice chest, 10° to 20° C.: 
7 days 
14 days 
— 
t 
“T 
- 
- 
19 days 
It will be seen from these experiments that the pathogenic bacteria 
usually die within a week or ten_ days in glycerin when exposed at 
37° C. in the incubator. At room temperature (22° C.) glycerin is 
