16 
[+ means growth; — means no growth.] 
Kept in— 
Filter paper. 
Distilled water. 
Per cent glycerin. 
Remarks. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.: 
7 days 
+ 
a+ 
+ 
+ 
-1- 
+ 
+ 
+ 
+ 
+ 
4- 
14 days 
+ 
+ 
+ 
+ 
+ 
+ 
-1- 
4- 
4- 
+ 
+ 
61 days 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
97 days 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
4 
122 days 
-f 
-1- 
+ 
+ 
+ 
+ 
b+ 
+ 
4- 
4- 
4- 
+ 
153 days 
-1- 
+ 
+ 
+ 
+ 
+ 
e+ 
+ 
-1- 
4 
+ 
+ 
184 days 
+ 
+ 
+ 
+ 
+ 
+ 
h+ 
4- 
+ 
4- 
4 
4- 
All cultures ivere of 
slow growth and 
rather scanty. 
215 days 
+ 
— 
— 
— 
— 
+ 
+ 
— 
fc+ 
— 
236 days 
+ 

n+ 

247 days 
Koom, 22° to24°C.: 
7 days 
-1- 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
+ 
14 days 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
4- 
61 days 
— 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
4- 
+ 
97 days 
+ 
+ 
-f 

4- 
-f 
4- 
-f 
4- 
4- 
4- 
122 days 
+ 
4- 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
+ 
153 days 
-1- 
-f 
-1- 
4- 
/+ 
4- 
4- 
4- 
4- 
184 days 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
4- 
4 
— slow and scanty 
growth. 
91 ft fi y<a 
4- 
l4- 
236 days 
+ 
0-j- 
247 days 
Ice chest,' 10° to 
12° C.: 
7 days 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
+ 
+ 
4- 
12 days 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
+ 
+ 
4- 
4- 
61 days 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
4 
4- 
97 days 
+ 
+ 
-f 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
4- 
4 
122 days 
+ 
+ 
+ 
+ 
+ 
+ 
4- 
+ 
4- 
4- 
153 davs 
+ 
+ 
+ 
+ 
+ 
+ 
0+ 
+ 
4- 
+ 
4- 
4 
184 days 
+ 
+ 
+ 
+ 
+ 
J+ 
4 
4- 
+ 
+ 
4 
— slow and scanty 
growth. 
215 days 
+ 
+ 
+ 
+ 
— 
+ 
— 
4- 
— 
+ 
236 days 
4- 
P+ 
4- 
4- 

4- 


247 days 
-j- 
-f 
3-i 
4- 
4- 
4- 
4- 
278 days 
+ 
r+ 
— 
+ 
— 
Material exhausted. 
M No growth first time; growth when replanted. 
(b) 0.5 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in six days. 
(c) 0.5 c. c. of the broth growth obtained from the 60 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(d) 0.5 c. c. of the broth growth obtained from the 70 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(e) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(/) 1 c. c. of the bouillon growth obtained from the 60 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(S') 1 c. c, of the bouillon growth obtained from the 50 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in twenty-four hours. 
(h) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in seventy-two hours. 
( i) 1 c. c. of the bouillon growth obtained from the 60 per cent glycerine kept at room temperature 
was inoculated subcutaneously in a mouse; dead in seventy-two hours. 
(J) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(k) 1 c. c. of the bouillon growth obtained from the 90 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in eighty-four hours. 
( ^) 1 c. c. of the bouillon growth obtained from the 90 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(»i) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the ice chest-was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(n) 1 c. c. of the bouillon growth obtained from the 60 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in sixty hours. 
(o) 1 c. c. of the bouillon growth obtained from the 90 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(P) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(3) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(r) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
