18 
BACILLUS TYPHOSUS. 
The various percentages of glycerin (S. ) mixed with distilled water were inoculated 
with a young culture of B. typhosus free of foreign organic matter, and then each 
flask containing the test fluid was thoroughly shaken so as to make as uniform an 
emulsion as possible. The shaking was repeated each time before the fluid was 
withdrawn for the purpose of making the counts. 
One series of flasks was kept in the ice chest at 10° to 12° C. and another in the 
incubator at 37° C. 
The figures represent the number of organisms (i. e., colonies) per cubic centi- 
meter. 
When 
counted. 
Distilled water. 
20 per cent 
glycerin. 
50 per cent 
glycerin. 
80 per cent 
glycerin. 
Pure glycerin. 
Incubator. 
Ice chest. 
Incu- 
bator. 
Ice 
chest. 
Incu- 
bator. 
Ice 
chest. 
Incu- 
bator. 
Ice 
chest. 
Incu- 
bator. 
Ice 
chest. 
At once 
1 dav 
2 days 
3 days 
4 days 
10, 710, 000 
5, 040, 000 
380, 000 
70. 000 
11. 000 
29 
11. 655. 000 
21. 630. 000 
9. 450. 000 
10. 710. 000 
10, 290, 000 
8. 400. 000 
4. 480. 000 
4. 410. 000 
4, 000, 000 
3, 860, 000 
4. 100. 000 
5. 080. 000 
452. 000 
600. 000 
180, 000 
150, 000 
118, 000 
26, 000 
500 
300 
80 
56 
115, 000 
86, 000 
1,000 
10 
28 
124, 000 
4.000 
1.000 
26 
3 
2, 000 
1, 000 
2 
3.000 
1.000 
4- 
2,000 
+ 
2,000 
-h 
5 days 
6 days 
7 days 
10 days 
13 days 
16 days 
21 days 
26 days 

31 days 

■ 
BACILLUS COLI COMWUXIS. 
The various percentages of glycerin (M. &R. ) mixed with distilled water were 
inoculated with a young culture of B. coll comrnunis free of foreign organic matter, 
and then each flask containing the test fluid was thoroughly shaken so as to make 
as uniform an emulsion as possible. The shaking was repeated each time before 
the fluid was withdrawn for the purpose of making the counts. 
One series of flasks was kept in the ice chest at 10° to 12° C. and another in the 
incubator at 37° C. 
The figures represent the number of organisms (i. e., colonies) per cubic centimeter. 
When counted. 
Distilled water. 
20 per cent glycerin. 
50 per cent glycerin. 
80 per cent 
glycerin. 
Incu- 
bator. 
Ice chest. 
Incu- 
bator. 
Ice chest. 
Incu- 
bator. 
Ice chest. 
Incu- 
bator. 
Ice 
chest. 
At once 
1 day 
2 days 
3 days 
4 days 
5 days 
6 days 
7 days 
8 days 
3.760.000 
9, 660, 000 
7, 728, 000 
6, 930, 000 
5, 670, 000 
6. 130. 000 
7. 630. 000 
4. 330. 000 
4. 250. 000 
3. 465. 000 
3. 523. 000 
2. 870. 000 
1. 600. 000 
880, 000 
515. 000 
480. 000 
390. 000 
340. 000 
370, 000 
280. 000 
102, 000 
104, 000 
8. 064. 000 
11. 708. 000 
6. 410. 000 
8. 190. 000 
9. 450. 000 
9. 210. 000 
7. 630. 000 
8. 400. 000 
9, 800, 000 
7, 770, 000 
7. 805. 000 
8,440, 000 
6.440.000 
1, 290, 000 
6. 300. 000 
5. 230. 000 
8. 400. 000 
4.410. 000 
4, 000, 000 
5. 200. 000 
7. 500. 000 
10. 080. 000 
4, 440, 000 
19,000 
3, 000 
84 
4 
2 
4, 680, 000 
131, 000 
56.000 
4, 200 
42.000 
51. 000 
10. 000 
2,000 
2,700 
1,000 
100 
100 
40 
9 
4 
8 
2, 416, 000 
4- 
-|- 
129 
1, 760, 000 
28, 000 
630, 000 
28, 000 
3, 000 
12, 600 
+ 
1 
500,000 
■f 
-|- 
420 
-1- 
100 
2 
664. 000 
181. 000 
14, 000 
17, 010 
111,000 
1,000 
-t- 
+ 
9 days 
10 days 
12 days 
14 days 
16 days 
17 days 
19 days 

21 days 
23 days 
24 days 
26 days 
29 days 

31 days 
