20 
The next series of experiments was designed to test the effect of 
glycerin upon the organisms of pus, both in pure culture and the pus 
itself. 
For this purpose pus was obtained from various sources, abscess of 
hand, axillary abscess, abscess of face, and a bone felon. The inflam- 
matory exudate was immediately planted in varying percentages of 
glycerin and examined from time to time. For comparison the micro- 
cocci were plated out in pure cultime and exposed to glycerin in like 
manner under exactly the same conditions. 
PUS — STAPBTVLOCOCCUS PYOGEXES AVRETS. 
Pus from an abscess of tbe hand, containing Staphylococcus pyogenes aureus in pure 
culture, planted in vaiAung percentages of glycerin (G) in distilled water and kept in 
the incubator at 37° C. From time to time small portions of the emulsion were drawn 
off and planted upon agar and in bouillon. 
Days. 
Percentage of glycerin. 
Remarks. 
10 20 
30 40 
50 60 
70 
80 
90 
100 
7 
14 
21 
^ ' 
-!_ ' 
' ; 
All marked — gave no growtli 
in broth. 
28 
3o 
_i_ 
42 
■ 
STAPHYLOCOCCUS IX PUEE CUXTUEE FEOM THE ABOVE PUS. 
A pure culture of Staphylococcus pyogenes aureus isolated from the above pus obtained 
from abscess of the hand was planted in various percentages of glycerin and tested as 
above. 
Days. 
Percentage of glycerin. 
Remarks. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
7.-.------. 
-r 
A- 
“T 
+ 
- 
— 
— 
All marked — gave no growth 
in broth. 
14 
21 
28 
35 
42 
49 


