24 
The toxin and the watery suspension of spores were then tested separately on mice 
as indicated by the following table: 
Result. 
Inoculated 
into mice — 
Tetanus toxin 
Tetanus spores. 
Jan. 22: 
0. 00006 c. c. 
.0002 c. c.. 
.0004 c. C-. 
Jan. 23-27, N. 
Jan. 23, N 
Jan. 23, N 
Jan. 28-30, P. 
Jan. 24, P ... 
Jan. 24-28, P. 
Recovered.. 
Jan. 25, dead 
Jan. 29, dead 
Jan. 23, N . 
Jan. 23, N . 
Jan. 23, N . 
Jan. 24-30, P. 
Jan. 24-31, P . 
Jan. 24-29, P . 
Jan. 31, recovered. 
Jan. 31, killed. 
Jan. 30, killed. 
Six sets of tubes were prepared, each set composed of eleven tubes, 
each tube containing 10 c. c. of fluid. The first tube of each set con- 
tained distilled water; the second contained 10 per cent pure glycerin 
in distilled water; the other tubes contained 20 per cent, 30 per cent, 
etc., to 100 per cent. 0.5 c. c. of toxin was added to each tube of 
three sets and the suspension of spores was distributed evenly among 
the other three sets, each tube getting 0.5 c. c. A set of spores and a 
set of toxin were placed at room temperature; a set of each was kept 
in the cool chamber and the other two sets were put in the incubator 
at 37° C. 
On February 1 these tubes were tested on mice as shown in the fol- 
lowing tables, having been two days in glycerin, and thereafter each 
month. 
TETANUS SPORES IN GLYCERIN. 
The following tables give the results of our studies of tetanus 
spores, washed free of toxin, in varying percentages of glycerin, and 
at difl'erent temperatures: 
Tetanus spores. 
AFTER TWO DAYS’ EXPOSURE TO GLYCERIN. 
[+ = typical symptoms of tetanus; — = no symptoms of tetanus; k = killed; d = died.] 
Quantity inoculated into mice, per- 
centages of glycerin, and temper- 
ature. 
Result. 
1st 2d 
day. day. 
3d 
day. 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
11th 
day. 
12th 
day. 
Incubator, 37° C.: 
0. 05 c. c. distilled vater 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
-t= 
+ 
+ + + + + + +4-4- + + -H + + + + + -1- 
+k 
20 per cent glvcerin 
40 per cent glvcerin 
+k 
60 per cent glycerin 
80 per cent glvcerin 
+k 
+ 
+k 
100 per cent glycerin 
Ice chest, 10° to 12° C. : 
0. 05 c. c. distilled water 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
+ + + + + + -h-h 
100 per cent glycerin 
Room, about 20° C.: 
0. 02 c. c. distilled water 
4- 
+k 
+ 
+k 
+k 
+ 
+k 
20 per cent glycerin 
... 
40 per cent glycerin 
+k 
60 per cent glvcerin 
80 per cent glycerin 
100 per cent glycerin 
+k 
