25 
Tetanus spores — Continued. 
AFTER THIRTY DAYS’ EXPOSURE TO GLYCERIN. 
[+ = t 3 rpical symptoms of tetanus; — = no sjTnptoms of tetanus; k = killed; d = died.] 
Quantity inoculated into mice, per- 
centages of glycerin, and temper- 
ature. 
Incubator, 37° C.’ 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Ice chest, 10° to 12° C.: 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Room, about 20° C.: 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Result. 
1st 
day. 
2d 
day. 
3d 
day 
4th 
day. 
5th j 6th 
day.j day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
11th 
day. 
12th 
day. 
— 
-1- 
+k 
+ 
+k 
+k 
+k 
+k 
+ 
+ 
+ k 
— 
- 
- 
- 
— 
— 
- 
— 
- 
+k 
+k 
+k 
-Ik 
AFTER SIXTY DAYS’ EXPOSURE TO GLYCERIN. 
Incubator 37° C.: 
n r> n Hiafillarl wafor 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
Ice chest, 10° to 12° C.: 
0.05 c. c. distilled water 
— 
1 II 1 1 1 1 4- + -f-I+ 1 1 II 1 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
■ 
100 per cent glycerin. . . ; . . 
Room, about 20° C.: 
0.05 c. c. distilled water 
+ 
+ 
+ 
+ 
-f 
+k 
+k 
-fk 
+k 
+k 
+ 
+ 
4- 
+ 
+ 
+ 
+ 
-I 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
AFTER NINETY DAYS’ EXPOSURE TO GLY'CERIN. 
Incubator, 37° C.: 
0.05 c. c. distilled water 
10 ner cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Ice chest, 10° to 12° C.: ” 
0.05 c. c. distilled water 
10 per cent glycerin 
- 
+ + + + 1 
-I 
4-k 
+k 
+ 
+ 
+ k 
30 per cent glycerin 
* 50 per cent glycerin 
70 per cent glycerin 
+ k 
+ k 
90 per cent glycerin 

Room, about 20° C.: 
0.05 c. c. distilled water 
10 per cent glycerin 
30 per cent glycerin 
— 
— 
+ 
-I 
1 1 -I-+ 1 
It 
+k 
— 
— 
50 per cent glycerin - 
70 per cent glycerin 
90 per cent glycerin 
- 
