27 
Tetanus spores. 
AFTER ONE HUNDRED AND TWENTY DAYS’ EXPOSURE TO GLYCERIN. 
[+ = typical symptoms of tetanus; — = no symptoms of tetanus; k = killed; d = died.] 
Quantity inoculated into mice, per- 
centage of glycerin, and tempera- 
ture. 
Result. 
1st 
day. 
2d 
day. 
3d 
day. 
4tb 
day. 
5tb 
day. 
6th 
day. 
7tb 
day. 
8tb 
day. 
9th 
day. 
10th 
day. 
11th 
day. 
12th 
day. 
Incubator, 37° C.: 
0.05 c. c. distilled water 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
Ice chest, 10° to 12° C. : ” 
0.05 c. c. distilled water 
20 per cent glycerin 
+ 
+ 
? 
? 
+k 
+k 
+ 
40 per cent glycerin 
60 per cent glycerin 
+ 
+ 
+k 
80 per cent glycerin 
100 per cent glycerin 
Room, about 20° C.: 
0.05 c. c. distilled water 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
AFTER ONE HUNDRED AND FIFTY DAYS’ EXPOSURE TO GLYCERIN. 
Ice chest, 10° to 12° C.: 
0. 05 c. c. 10 per cent glycerin 

-f- 
+ 
+ k 
30 per cent glycerin 
+ k 
50 per cent glycerin 
+ k 
70 per cent glycerin 
+ 
+ 
+ k 
90 per cent glycerin 
Room, about 20° C.: 
0. 05 c. c. 10 per cent glycerin 
30 per cent glycerin. . . 
~ 
— 
+ 
+ 
+ 
+ k 
50 per cent glycerin 
70 per cent glycerin 
■ 90 per cent glycerin 
AFTER ONE HUNDRED AND EIGHTY DAYS’ EXPOSURE TO GLYCERIN. 
Ice chest, 10° to 12° C.: 
0. 05 c. c. 20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
Room, about 20° C.: 
0. 05 c. c. 20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 

? 
+k 
+ 
+k 
+k 
? 
1 1 + 1 1 11 
— 
- 
- 
- 
— 
— 


- 
= 
— 
- 
— 
We learn from these studies that tetanus spores may die within 
thirty days in glycerin at the body temperature; but they live for 
months (one hundred and eighty days) at room temperature or in the 
ice chest. The virulence of the spores is generally lost long before 
their power of growing and multiplying if placed under favorable 
conditions. 
