44 
against the immunity unit every week or two in order to assure oui- 
selves that they are not undergoing change. A number of carefully 
tested toxines are always kept on hand, so that we may have a double 
test of the serums we are standardizing, as well as to guarantee against 
changes in the toxines which mav set in suddenlv. 
The exact standardization of a toxine takes considerable time and 
we therefore can not depend upon one poison. The exact L+ dose 
of a well-seasoned toxine is the keynote of the whole testing opera- 
tion. Without this test dose good results are impossible. Every 
effort must be made to obtain a reliable poison that answers all the 
requirements and, having such a toxine, to maintain it under the most 
careful conditions in order to keep it in a state of equilibrium as long 
as possible. As the toxine grows old the tests naturally multiply in 
number and increase its reliability for the purpose of determining the 
unit strength of antitoxins. 
On account of the importance of the L+ dose I exhibit here a series 
of L+ tests showing the character of the results obtained bv the 
poison after it has gained its stage of equilibrium. 
Table ghing the results of tests on Toxine Xo. 7 for L— dose, using O.Sl c. c. of the toxine 
against one immunity unit, from October 24, 1904, to December 22, 1904. 
Guinea pig 
No. 
Time of 
death. 
Eemarks. 
Guinea pig 
No. 
Time of 
death. 
Remarks. 
Dys. hrs. 
1 
Dys. hrs. 
395 
3 
0 
1 
859 
2 
20 
Usual reactions. 
453 
3 
5 
1 
860 
9 
15 
Do. 
454 
5 
15 
903 
3 
7 
544 
3 
9 
i 
904 
3 
16 
545 
3 
21 
905 
6 
2 
546 
3 
5 
948 
3 
9 
547 
3 
18 
949 
3 
2 
601 
5 
16 
950 
3 
18 
602 
9 
17 
Clear serous fluid in pleu- 
951 
3 
18 
ral cavities. 
984 
3 
6 
603 
3 
13 
985 
1 
18 
Pneumonia. 
604 
3 
6 
986 
1 
17 
Peritonitis; serous exu- 
605 
1 
18 
Omentum, mesentery, and 
date; omentum very red. 
broad ligament inject- i 
987 
5 
6 
ed, etc. ' 
988 
1 
17 
Pneumonia. 
621 
3 
9 
989 
9 
6 
Clear serum in pleural 
622 
2 
19 
Clear fluid in pleural cav- 
- 
cavities. 
itv. 
1063 
4 
20 
623 
3 
9 
I 
1064 
3 
0 
624 
3 
14 
: 
1065 
3 
12 
625 
3 
7 
1066 
4 
13 
693 
3 
18 
1067 
3 
8 
694 
4 
11 
1 
1068 
3 
4 
695 
3 
14 
1069 
1 
17 
Pneumonia, both upper 
696 
4 
8 
lobes. 
697 
3 
16 
i 
1124 
2 
17 
Spleen and liver show 
750 
3 
19 
sharply defined caseous 
751 
2 
12 
Peritoneum discolored 
areas. 
and petechise through- 
1125 
4 
16 
out. 
1126 
3 
17 
752 
2 
12 
Some peritoneal reaction 
1127 
5 
17 
and clear serum in pleu- 
1128 
2 
17 
Usual reaction. 
rse. 
1129 
3 
6 
753 
2 
14 
Nothing unusual, adre- 
1150 
3 
8 
nals (Tongested, local re- 
1151 
1 
19 
Severe peritoneal reac- 
action. 
tion. 
754 
■2 
17 
Do. 
1152 
2 
17 
Peritoneal fat injected. 
808 
3 
17 
1153 
3 
11 
809 
4 
14 
1154 
2 
16 
Clear serum in pleural 
855 
2 
20 
Usual reaction. 
cavities. 
856 
4 
4 
1155 
2 
12 
Usual reaction. 
857 
3 
9 
1156 
3 
11 
858 
4 
4 
1 
1 
