45 
r During- two months covered b}" the table every one of -the 68 
animals injected with 0.21 c. c. of this toxine, mixed with 1 immunity 
unit, died acutel}". None recovered. Most of the guinea pigs died on 
the fourth day. As we usually inoculate our animals at about 3 o’clock 
in the afternoon, animals d}dng between three days two hours and 
four days two hours will appear on the autopsy table on the fourth 
dav. 
Animals dying before the fourth da}" usually show at autopsy some 
reason for this increase of susceptibility. 
The following tables used for diluting our toxines for MLD and L+ 
doses will be found useful: 
TABLES FOR DILVTIXG TOXINES FOR MLD. 
First dilution. 
1+99 = 1:100 
Second dilution. 
1 of first dilution+49 = l: 5000 
1 of first dilution+39 = l: 4000 
1 of first dilution+29 = l: 3000 
1 of first dilution+9 = 1:1000 
1 
c. 
c.=.0002 
1.5 
c. 
c.=.0003 
2 
c. 
c.=.0004 
2.5 
c. 
c. = .0005 
3 
c. 
c.=.0006 
0.8 
c. 
c.=.0002 
1 
c. 
c. = .00025 
1.2 
c. 
c.=.0003 
1.6 
c. 
c. = .0004 
2 
c. 
c. = .0005 
2.4 
c. 
c. = .0006 
2.8 
c. 
c. = .0007 
3.2 
0- 
c. = .0008 
0.9 
c. 
c. = .0003 
1 
c. 
c. = .00033 
1.2 
c. 
c.=.0004 
1.5 
c. 
c. = .0005 
1.8 
c. 
c. = .0006 
2.1 
c. 
c. = .0007 
2.4 c. 
c. = . 0008 
2.7 
c. 
c. = .0009 
3 
c. 
c. = .001 
1. 
c. 
c. = .001 
1.5 
c. 
c. = .0015 
2 
c. 
c. = .002 
2.5 
c. 
c. = .0025 
3 
c. 
c. = .003 
