8 
The following table shows the results of his experiments with 
anthrax spores exposed in greater percentages of zinc chloride: 
Time of exposure in davs. 
Pereentasre ol zine 
chloride. 1 ■ 3 5 10 
20 ; 30 
5 -f — ; -r -r 
9 _L- _i_ 1 ^ 
1 i 
t { 
The mark -f indicates that the spores taken from the zinc chloride 
solution on the respective days had undiminished power to develop. 
Anthrax spores exposed on silk threads in a percentage of 1:200 of 
zinc chloride dissolved in blood serum retained their power to develop 
after dS hours' exposure equally as well as the controls. This he 
observed under the microscope. 
From his experiments he concludes that ZnClg in a percentage of 
1:20 has no power to influence anthrax spores, so far as their develop- 
ment is concerned, after an exposure of one week. 
He further concludes that it is a fact that zinc chloride has no 
marked power to prevent micro-organic growth, and that it is, indeed, 
incomprehensible to him that any considerable disinfecting value 
could be ascribed to this substance. 
F. Bouillat^' recognized zinc chloride as a good antiseptic, but found 
that a 5 per cent solution did not kill the spores of anthrax. To blood 
seiuun and egg albumen diluted with two to four times their volume 
of water he added zinc chloride and observed that it coagulated the 
albumen, forming an insoluble zinc albuminate, and that, provided 
enough of the salt be added to unite with the whole of the albumen, 
no growth can take place. In his experiments with this zinc albumi- 
nate he also observed that it has some power for inhibiting bacterial 
growth and the development of foul odors. 
MiqueF placed chloride of zinc in his class 3 of substances as 
■’strongH antiseptic" and states that 1.9 parts in 1.000 will prevent 
putrefaction in neutral beef bouillon. He places it in the same class 
with permanganate of potash, chloroform, cyanide of potash and car- 
bolic acid. He also states that 1.9 parts of zinc chloride is equivalent 
to 3.2 parts of carbolic acid for preventing putrefactive changes in 
neutral beef bouillon. 
Sternberg^ says: **In the writer's experiments 1:200 destroyed 
Jlic'rococcus Pasteuri in 2 hours, but a 2 per cent solution was required 
to kill pus cocci in the same time: spores of Bacillus anthracis were 
not destroyed by 2 hours' exposure in a 10 per cent solution, but a 
«Beitriige ziir lehre von der antisepsis, 1882. 
^Les organismes A'ivants de Tatinosi^here. 1883. 
c Text-book of bacteriology, 1891, p. 195. 
