5 
Table IT. 
Bouillon with the addition of ferrous sulphate in various percentages contaminated 
i by the addition of a small quantity of garden earth. Kept in the incubator. 
^1 ^ ^ 
Proportion of fer- 
rous sulphate. 
First day. 
Second day. 
Third day. 
Fifth day. 
Fourteenth 
day. 
1 • 9on 
No odor. 
J. . ZuU 
1:150 
1 • inn 
Foul odor 
tin 
1 • ^n 
odor 
Foul odor .... 
1 
1 . OU 
rio 
do 
± , oO 
1 • ^n 
do 
do 
1 
1 • 9^ 
rio 
No odor 
Foul odor 
1 . ZO 
1 -9n 
do 
do 
No odor 
Foul odor 
do 
No odor 
1-9?? 
do . . . ^ 
do 
hbb 
O O 
1-90 
do 
do 
GERMICIDAL ACTION OF FERROUS SULPHATE. 
: I Forty-eight hour bouillon cultures of B. typhosus, B. coli communis, B. pyocyaneus, 
II vibrio cholera Asiat., strong aqueous suspensions from forty-eight hour cultures on 
' agar of B. anthracis, B. subtilis, Staphyl. pyog. aureus and albus, and a strong sus- 
pension of a forty-eight hour growth of B. diphtheria on blood serum were used to 
contaminate slips of filter paper, silk threads, and glass cover slips. After being con- 
^ . taminated by the culture the paper slips, silk thread and cover slips were placed in 
I, the incubator until they were almost dry (in the case of anthrax and subtilis they 
i , were allowed to dry completely), then the disinfecting agent was applied for definite 
periods, its action stopped by careful washing in sterile water, the slips and threads 
planted in bouillon and placed in the incubator for forty-eight hours. 
Table III . — Infected material, filter paper. 
Result after application of sat. sol. of ferrous sulphate. 
Organism employed. 
1 5 minutes. 
10 minutes. ' 
20 minutes. 30 minutes. 
45 minutes. 
60 minutes. 
B. anthracis 
-F 
+ 
+ 
+ 
+ 
4- 
B. subtilis 
+ 
+ 
-f- 
+ 
4- 
4- 
B. tvphosus 
-f- 
+ 
-r 
4- 
4- 
B. coli communis 
.' 4- 
+ 
+ 
-f 
4- 
4- 
B. pvocvaneus 
-1- 
-4 
4 
1 4- 
Yib. cholera Asiat 
-s 
-1- 
+ 
+ 
4- 
1 4- 
B. diphtheria 
-r 
i -f 
+ 
-f 
4- 
■ 4- 
Staphvl. pvog. aureus 
O- 
1 + 
-1- 
+ 
4- 
4- 
Staphvl. epider. albus 
- 
1 + 
-t- 
4- 
4- 
i 4- 
{ . Table IV . — Infected material, silk thread^. 
Result after application of saturated solution of ferrous sulphate. 
Organism employed. 
5 minutes. 
10 minutes. 
20 minutes. 30 minutes, j 
45 minutes. 60 minutes. 
B. anthracis ' 
j 
+ > 
- 1 
-r 4- 
B. subtilis 
; 
4 - 
— -T 
4- 4- 
B. tvphosus 
— 
4- 
•V — 
4- 
B. coli communis 
-r 
4- 
-T -f 
T ' 4- 
B. pvocvaneus 
-r 
-h 
~r “T 
4- 
B. diphtherise 
+ 
4- 
+ + 
4- 4- 
Vibrio cholera Asiat 
+ 
4- 
+ 
4- 4- 
Staphyl. pyogenes aureus 
-f 
4- 
+ -f- 
1 T 
Staphvl. epider. albus 
* 
1 ^ 
