10 
Nutrient bouillon containing various 'percentages of glycerin {S.), contaminated by the 
addition of stable manure, and kept in flasks at room temperature. 
Percentage of glycerin. 
Day upon which gro^vth appeared. 
Ninth day. 
Tenth day. 
Thirteenth day. 
Twenty-first 
day. 
41 per cent 
Small surface 
mold. 
No growth. 
Do. 
42 per cent 
Small surface 
mold. 
Very small sur- 
face mold. 
43 per cent 
44 per cent 
Small surface 
mold. 
45 per cent 
46 per cent ' 
As was to be expected, the exact percentage of glycerin necessaiy to 
restrain growth varies within narrow limits with the make of glycerin 
used and the kind of contamination. 
To sum up the antiseptic power of glycerin: 
Per cent. 
Glycerin X 49 
Glycerin M 45 
Glycerin P 43 
Glycerin S 45 
Average 45. 5 
These results do not correspond with Miquel’s work in 1881, who 
found that 225 grams of gl 3 "cerin in 1 liter of bouillon was sufficient 
to prevent putrefaction. 
The above test having determined the antiseptic power of glycerin 
against putrefactive changes, another series of experiments was made 
in order to determine the restraining power of gtycerin against pure 
cultures of various micro-organisms. 
The different percentages of gl^^cerin were made as before with 
nutrient bouillon and this time distributed into test tubes. Each tube 
was inoculated with a minute but visible portion of the surface growth 
of the organisms, which were grown for this purpose upon agar slants 
under favorable conditions, and fresh young cultures were alwa^^s used 
to make the inoculation. 
The test tubes were incubated at 31° C. and examined daity for 
growth. In case a growth appeared it was tested for purit 3 ^ The 
tubes Avere observed dail}" for at least eleven days and the result noted. 
