11 
As a result of the foregoing experiments glycerin is found to be 
antiseptic in the following dilutions: 
Organism. 
Glycerin. 
Average 
percent- 
age. 
P. 
X. 
S. 
G. 
Staphylococcus pyogenes aureus 
Per cent. 
30 
Per cent. 
32 
Per cent. 
33 
Per cent. 
29 
Per cent. 
31 
Staphylococcus pyogenes citreus 
27 
25 
26 
26 
27 
32 
31 
30 
27 
32 
28 
27 
28.5 
Bacillus typhosus 
25 
23 
25 
25 
24.5 
Bacillus coli communis 
28 
26 
24 
27 
26. 25 
Bacillus icteroides 
26 
25 
23 
24 
24.5 
Bacillus acidi lax^tici 
27 
27 
25 
25 
25 
25.5 
Bacillus enteritidis 
24 
23 
23 
24. 25 
Bacillus dysenterise 
27 
22 
24 
23 
24 
Vibrio cholerse 
21 
21 
21 
24 
21.75 
Bacillus diphtherise 
25 
22 
23 
23 
23. 25 
Bacillus anthracis 
31 
26 
26 
31 
28.5 
Bacillus pestis 
23 
21 
21 
22 
21.75 
Bacillus glanders 
23 
23 
24 
24 
23.5 
Bacillus pyocyaneus 
25 
22 
21 
25 
23. 25 
Bacillus subtilis 
26 
24 
21 
27 
24.5 
Bacillus proteus vulgaris 
27 
22 
22 
21 
23 
Bacillus megaterium 
20 
20 
15 
20 
18. 75 
Bacillus prodigiosis 
20 
21 
20 
20 
20.25 
Bacillus flourescens liquefaciens 
23 
21 
21 
20 
21. 25 
Averages 
25.3 
24.1 
23.7 
24.6 
24.4 
It was seen from a stud}^ of the results obtained that the antiseptic 
power of glycerin varies with the organism and also with the kind of 
ghmerin used. For example, the growth of cholera and plague is 
retarded by the presence of 21 to 21 per cent of glycerin, while pus 
cocci will grow in 31 per cent. The antiseptic power of glycerin 
‘‘X” and ‘‘S” is greater than that of “P” or “G.” 
It was also seen that the molds and common bacteria of putrefaction 
grow in bouillon containing between 10 and 50 per cent of glycerin, 
while the pure culture of the bacteria tested do not grow in percent- 
ages above 31. 
Attention is especially called to the interesting fact brought out by 
this series of tests that the pus cocci are able to grow and multipl}^ in 
higher percentages of gl 3 "cerin than any of the other 18 micro-organisms 
tested. 
THE GEKMICIDAL POWER OF GLYCERIN. 
The following tests were made to determine the time required for 
glycerin to destro}^ the important pathogenic organisms. The work 
included tests with various percentages of gl}merin at different tem- 
peratures. Our object was to determine the exact effect of gljmerin 
free from all other substances upon pure cultures. Therefore the 
bacteria were grown upon agar slants and carefull}^ taken from the 
surface, so as to obtain the colonies free from the organic matter on 
which they rested. 
STAPHYLOCOCCUS PYOGENES AUREUS. 
The various percentages of glycerin were made with sterile distilled water and 
distributed into test tubes. Each tube was abundantly inoculated with a young cul- 
