12 
ture of Staphylococcus pyogenes aureus taken from the surface of an agar slant free of 
foreign organic matter. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c.) of the test fluids and also 
one of the slips of filter paper was planted in bouillon. 
[+ means growth; — means no growth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
I Per cent glycerin. 
i 
20 
30 
40 
50 
1 60 
70 
80 
90 
100 
cubator, 37° C.: 
7 days 
+ 
+ 
— 
i — 
— 
— 
+ 
— 
_ 
.p 
_ 
13 daws 
Or\ Q V'G 
+ 
- 
- 
- 
- 
- 
- 
- 
- 
- 
ZU Ua \ 5 
27 days 
+ 
+ 
i - 
34 days 
41 days 
T 
1 
1 
48 days 
+ 
1 
I**" 
55 days 
1 
)Om, 22° to 24° C.: 
i 
7 days 
+ 
-e I 
+ 
-1- 
+ 
4- 
1 ~ 
+ 
+ 
+ 
+ 
— 
13 days 
+ 
+ 1 
-1- 
-f 
__ 
— 
_ 
— 
— 
20 days 
-1- 
+ ; 
— 
— 
— 
— 
1 _ 
- 1 
- 
— 
_ 
27 days 
+ 
! 
1 
1 
34 days ; 
+ 
T 
! 
.... 
I"" 
41 days ‘ 
3 chest* 10° to 12° C.: ' 
7 days 
+ 
-j- 
+ 
-P 
' 4- 
-1- 
+ 
+ 
-1- 
+ 
+ 
13 days ; 
+ 
+ 1 
+ 
-f 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
20 days ' 
-f 
+ 
+ 
+ 
— 
— 
-P 
+ 
+ 
.p ! 
27 days ^ 
_j_ 
-i- 1 
-p 


-p 
_i_ ' 
34 days ' 
+ 
-|- ! 
-p 
-p 
_L 
41 days 
1 
48 days 
+ 
1 
T 
i 1 
55 days I 
-i- 
-j- 
1 
62 days 
+ 
1 
69 days 
1 
1 
76 days i 
-!_ 1 
. .1 
j 
83 days ' 
+ 1 
90 days 
+ ! 
1 
1 
97 days ■ 
1 
111 days 
+ j 
....1 
1 
i 
1 
1 
1 
STAPHYLOCOCCUS EPIDERMIS ALDUS. 
The various percentages of glycerin were made with sterile distilled water and 
distributed into test tubes. Each tube was abundantly inoculated with a young 
culture of Staphylococcus epidermis albus, taken from the surface of an agar slant, free 
of foreign organic matter. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c. ) of the test fluids and also one 
of the slips of filter paper were planted in bouillon. 
