13 
[+ means growth; - means no growth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
Per cent glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.: 
7 days 
+ 
— 
— 
4 - 
— 
— 
— 

_ 
13 days 
+ 
— 
— 
— 
— 1 
— 
— 
— 
— 
_ 
20 days 
4- 
27 days 
- 
34 days 
-1- 
41 days 
+ 
48 days 
Room, 22° to 24° C.: 
7 days 
IQ Q TTO 
- 
+ 
- 
+ 
- 
- 
+ 
+ 
- 
+ 
+ 
- 
±o U-ciy 
20 days 
+ 
Ice chest, 10° to 12° C.: 
7 days 
+ 
+ 
+ 
— 
-h 
— 
— 
+ 
+ 
— 
— 
13 days 
+ 
+ 
+ 
— 
+ 
— 
— 
— 
— 
— 
— 
20 days 

27 days 
+ 
1 
34 days 
+ 
41 days 
+ 
48 days 
+ 
55 days 
+ 
62 days 
+ 
69 days 
BACILLUS COLI COMMUNIS. 
The various percentages of glycerin were made with sterile distilled water and 
distributed into test tubes. Each tube was abundantly inoculated with a young 
culture of B. coli communis^ taken from the surface of an agar slant, free of foreign 
organic matter. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c.) of the test fiuids and also one 
of the slips of filter paper were planted in bouillon. 
[+ means growth; — means no growth.] 
Kept in — 
Filter 
paper. 
Dis- 
tilled 
water. 
Per cent glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.; 
7 days 
+ 
+ 
-1- 
+ 
— 
— 
4- 
+ 
4- 
+ 
+ 
— 
14 days 
+ 
-1- 
4- 
— 
— 
— 
+ 
+ 
+ 
4- 
-f- 
— 
19 days 
— 
+ 
+ 
— 
— 
— 
— 
-h 
4- 
— 
— 
— 
29 days 
4- 
4- 
days . _ 
-f 
4- 
42 days . 
+ 
4- 
49 days 
4- 
4- 
56 days 
4- 
4- 
63 days 
4- 
4- 
1 . 
70 days 
4- 
4- 
77 days 
4- 
4- 
84 davs . 
4- 
4- 
91 days 
4- 
4- 
1 
98 days 
4- 
4- 
112 davs 
4- 
4- 
133 days 
4- 
154 days 
Room, 22° to 24° C. ; 
7 days 
— 
4- 
4- 
4- 
4- 
+ 
-f 
+ 
4- 
— 
+ 
+ 
14 days 
— 
4- 
+ 
+ 
+ 
-b 
-h 
+ 
4- 
-f 
— 
-t 
19 days 
— 
4- 
4- 
— 
— 
— 
— 
+ 
— 
— 
— 
— 
29 days 

4- 
4- 

.3,5 days. ... 
4- 
4- 
42 days 
4- 
49 days 
4- 
56 days 
4- 
