14 
[+ means growth; — means no groAvth.] 
Kept in — 
Filter 
paper. 
i 
Dis- 1 
tilled 1 
water. | 
Per cent glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Room, 22° to 24° C.— Continued. 
63 davs 
t 
' + 
; + 
_u 
i - 
1 + 
1 -1- 
-1- 
+ 
+ 
+ 
-h 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
! 
84 davs 
98 days 
112 days 
133 days 
Ice chest, 10° to 12° C.; 
7 days 
14 days 
19 days 
29 days 
35 days 
1 ++++++ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ ; 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
: -i- : 
+ ‘ 
+ 
+ 
4- 
; + 
^ + ■ 
-f ’ 
! + ! 
-1- 
-i- 
I + 
-i- 
+ 
+ 
(«) 
-t- 
+ 
+ 
ii 
_ i 
....j 
_I_ 
~r 
+ 
1 
+ 
4- 
42 days 
49 days 
56 days 
1.... 
63 days 
: ! 
1 
70 days 
77 days 
84 days 
91 days 
1 
98 days 
i 
112 days 
L... 
133 days 
1 
154 days 
i 
! 
a Slow. b Material exhausted. 
DIPHTHEEIA. 
The various percentages of glycerin were made with sterile distilled water and dis- 
tributed into test tubes. Each tube was abundantly inoculated with a young culture 
of dipththeria, taken from the surface of an agar slant, free of foreign organic matter. 
For comparison, the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
From time to time a small quantity (about 0.5 c. c. ) of the test fiuids and also one 
of the slips of filter paper were planted in bouillon. 
[+ means growth; — means no growth.] 
Kept in— 
Filter 
paper. 
Dis- 1 
tilled , 
water. , 
Per cent glycerin. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
Incubator, 37° C.: 
7 days 
- 
+ i 
! 
14 days 
19 days 
....j 
Room, 22° to 24° C.: 
7 days 
- 
1 
i 
+ i 
+ 1 
- 
-1- 
- 
- 
- 
- 
- 
- 
- 
- 
14 days 
19 days 
29 days 
Ice chesfi 10° to 20° C.: 
7 days 
- 
-h 
j 
1 
14 days 
19 davs 
1 
1 
i 
i 
It will be seen from these experiments that the pathogenic bacteria 
usuallv die within a week or ten days in glycerin when exposed at 
37° C. in the incubator. At room temperature (22° C.) glycerin is 
