15 
not so active, the organisms sometimes surviving two weeks. In the 
ice chest at 10° to 12° C. the germicidal power of glycerin is markedly 
diminished. Pus cocci live for several weeks, the colon bacillus sev- 
eral months. 
It will be noted from these tables that 50 per cent glycerin seems to 
be more actively germicidal than either stronger or weaker solutions. 
The next experiment was made to test the effect of glycerin upon 
spores, with the following results: 
BACILLUS ANTHRACIS. 
The various percentages of glycerin were made with sterile distilled water and dis- 
tributed into test tubes. Each tube was abundantly inoculated with a growth of 
B. anthracis containing many spores taken from the surface of peptoneless agar, care 
being taken to carry over only a culture free from the organic matter of the media 
used. 
For comparison the organism was also inoculated into sterile distilled water and 
upon sterile slips of filter paper. 
Three sets of tubes were made and kept at different temperatures, as indicated on 
the table. 
^ From time to time a small quantity (about 0.5 c. c. ) of the test fluids, and also one 
of the slips of Alter paper, were planted in bouillon. In adition to testing the vege- 
tability of the organism in this manner its virulence was tested from time to time as 
indicated on the tables by inoculation into mice. 
