16 
• 1 
[-i- means growth; — means no growth.] 
Kept in— 
I 
Per cent glycerin. 
Remarks. 
10 
20 30 
40 
50 
60 
70 
1 80 
[ 90 
100 
Incubator, 37° C.: 
1 
i 
7 davs 
“T 
a-f 
-r ' -f 
4_ 
4_ 
4- 
i 4- 
+ 
1 -r 
4- 
14 davs ...... 
4- 
4- 
-f i + 
4- 
4- 
! 
4* ' 
! 4- 
4- 
61 davs 
-f- 
-f 
“T 
-r 1 -f 
4- 
4- 
-i- 
1 4- 
4_ 
4- 
4_ 
97 davs 
— 
4_ 
-f 4- 
4- 
+ 
4- 
: 4- 
-f 
4- 
+ 
122 davs 
_ 
4- 
4- 
+ ■ -f 
4- 
&4- 
-f 
4* 
4- 
-f 
4- 
153 davs 
_ 
4- 
4- 
+ i + 
4- 
64- 
4_ 
4- 
4- 
4- 
1&4 davs 
— 
-L- 
-f ' -h 
4_ 
ft+ 
4~ 
-U i 
AR cultures were of 
slow growth and 
rather scanty. 
215 davs 
_ 
_ 

_ — 
— 
! — 
_ 
k~ 
— 
236 davs 

n— 
247 davs 
1 
Room. 22°'to24°C.: 
7 davs 
■ 1 
-f ■ + ’ 
^ -r 
4- 
.f 
-t 
a. 
4- 
4- 
14 days 
-1- 1 
1 
-f 
j_ _L 
-f 
4" 
-f 
4* 
-i- 
-r 
+ 
61 davs 
— 
i “T 
+ 
-h : 4- 
— 
+ 
-|- 
1 4- 
4- 
4- 
+ 
97 days 
I 4- 
-f- 
4_ __ 
_ 
4- 
4- 
1 4- 
4- 
4- 
4- 
122 davs 
— 
-f 
4- -r 
4- 
4- 
6-f 
4- 
4- 
+ 
1-53 davs 
1 
■ _ 
: 
4- 
/-L 
4- 
r 
4- 
+ 
184 days 
4- + 
_L 
4_ 
*■ + 
i 4- 
4_ 
— slow and scanty 
1 
growth. 
215 days 
■ 

\ — 
_ 
_ 
1 + 
4" 
236 davs 
1 
i ~ 
04- 
247 davs 
....1 
|.... 
Ice chest, 10° to 
1 

1 
1 
12° C.: 
i 
1 
! 
7 davs 
4- 
-h 
-r 
4- 
-r 
-r 
4- 
4- ! 
i 4- 
4* 
12 davs 
-j- 
-r 
-r -r 
4- 
‘ 4- 
4- 
4- 
+ 
61 davs 
1 
+ 
4- 4- 
4- 
4- 
: -b 
4- 
4" 
4- 
+ 
97 davs 
4- 
+ 
-r 
4- 4- 
-1- 
4- 
-j- 
4- 
4- 
4- 
4- 
122 davs 
-|- 
-j- 
+ 
+ 4- 
4- 
4- 
4- 
d-f 
4- 
4- 
4- 
153 davs 
! 4- 
4- 
-|- 
4- ^ 4- 
+ 
94- 
4- 
-f 
4- 
4- 
“T 
184 davs 
-4 
4- 
T 
4- -t- 
. 
J-r i 
4- 
4- 
_i_ ' 
4- 
4- 1 
— slow and scanty 
1 
growth. 
215 days 
4- 
+ 
-f- 
-r , — 
m-f- 
— 
-j- 
— 
4- 
-r 1 
236 davs 
4- 
P + 
-4- 

_j_ 

- 1 
247 davs 
9-1- 

278 davs 
- 
- 
- 
.u ■ 
- 
- 
Material exhausted. 
Xo growth first time; growth when replanted. 
(6) 0.5 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in six days. 
{c) 0.5 c. c. of the broth* growth obtained from the M per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight houK. 
(d) 0.5 c. c. of the broth growth obtained from the 70 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty -eight hours. 
(e) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(/) 1 c. c. of the houilloh growth obtained from the 60 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(a) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in twenty-four hours. 
(ft) 1 c. c. of the bouillon growth obtained from the *50 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in seventy-two hours. 
( 0 1 c. c. of the bouillon growth obtained from the 60 per cent glycerine kept at room temperature 
was inoculated subcutaneously in a mouse; dead in seventy-two hours. 
(j) 1 c. c. of the bouillon ^owth obtained from the 50 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(ft) 1 c. c. of the bouillon ^owth obtained from the 90 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in eighty-four hours. 
(01c. c. of the bouillon growth obtained from the 90 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(»i) 1 c. c. of the bouillon growth obtained from the 50 per cent glycerin kept in the ice chest w^ 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(«) 1 c. c. of the bouillon growth obtained from the 60 per cent glycerin kept in the incubator was 
inoculated subcutaneously in a mouse; dead in sixty hours. 
(o) 1 c. c. of the bouillon growth obtained from the 90 per cent glycerin kept at room temperature 
was inoculated subcutaneously in a mouse: dead in forty-eight houre. 
(p) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hotirs. 
(3) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
(r) 1 c. c. of the bouillon growth obtained from the 10 per cent glycerin kept in the ice chest was 
inoculated subcutaneously in a mouse; dead in forty-eight hours. 
