19 
BACILLUS COLI COMMUNIS. 
The various percentages of glycerin (P.) mixed with distilled water were inoculated 
with a young culture of B. coli communis free of foreign organic matter, and then each 
flask containing the test fluid was thoroughly shaken so as to make as uniform an 
emulsion as possible. The shaking was repeated each time before the fluid was with- 
drawn for the purpose of making the counts. 
One series of flasks was kept in the ice chest at 10° to 12° 0. and another in the 
incubator at 37° C. 
The figures represent the number of organisms (i. e., colonies) per cubic centimeter. 
When 
counted. 
Distilled water. 
20 per cent glycerin. 
50 per cent glyc- 
erin. 
80 per cent 
glycerin. 
Pure glycerin. 
Incuba- 
tor. 
Ice chest. 
Incuba- 
tor. 
Ice chest. 
Incu- 
bator. 
Ice chest. 
Incu- 
bator. 
Ice 
chest. 
Incu- 
bator. 
Ice 
chest. 
At once . . 
1 day 
2 days . . . 
3 days... 
4 days... 
5 days... 
6 days... 
7 days... 
8 days... 
9 days 
4. 410. 000 
4.427. 000 
4.200. 000 
18, 900, 000 
8. 922. 000 
8.443. 000 
7. 700. 000 
3. 500. 000 
Flask 
broken. 
20. 700. 000 
1,610,000 
7.560.000 
9. 520. 000 
7. 376. 000 
8. 400. 000 
8. 470. 000 
9.500.000 
11,000, 000 
12,000,000 
14. 000. 000 
17. 000, 000 
14.280.000 
10.710.000 
13. 860. 000 
10. 290. 000 
11. 600. 000 
9. 480. 000 
8. 640. 000 
3, 632,000 
3,000 
1 
11, 900, 000 
5.040.000 
4.550. 000 
4.410. 000 
2.800. 000 
110, 000 
8,000 
1,000 
600 
18 
24 
15 
5 
2 
210,000 
-b 
11 
1, 582, 000 
+ 
500,000 
15.000 
29, 200 
16.000 
22, 000 
3, 000 
900 
200 
4 
408, 000 
4,000 
400, 000 
1,000 
8,000 
10, 000 
1,000 
2,000 
+ 
93, 000 

1 
11 days 
13 days 
15 days 
! 
16 days 
18 days 
20 days 
24 days 
27 days . . . 
31 days... 
1 
1 
BACILLUS DIPHTHERIiE. 
The various percentages of glycerin (P. ) mixed with distilled water were inocu- 
lated with a young culture of B. diphtherix free of foreign organic matter, and then 
each flask containing the test fluid was shaken so as to make as uniform an emulsion 
as possible. The shaking was repeated each time before the fluid was withdrawn 
for the purpose of making the counts. 
One series of flasks was kept in the ice chest at 10° to 12° C. and another in the 
incubator at 37° C. 
The figures represent the number of organisms (i. e., colonies) per cubic centimeter. 
When 
counted. 
Distilled water. 
50 per cent 
glycerin. 
100 per cent 
glycerin. 
Remarks. 
Incuba- 
tor. 
Ice 
chest. 
Incuba- 
tor. 
Ice 
chest. 
Incuba- 
tor. 
Ice 
chest. 
At once 
1,080 
2,450 
10 
20 
10 
1 dav 
0 
0 
1 
0 
0 
1 
All sterile in bouillon. 
Agar. 
Agar. 
2 days 
0 
0 
0 
0 
0 
0 
In bouillon. 
It is again evident from these experiments that the pus cocci in pure 
culture are destroyed by the action of glycerin within two weeks. 
Glycerin seems to be an active poison for the bacillus of diphtheria 
throughout all our experiments. 
The organisms belonging to the typhoid and colon group sometimes 
show a more, marked resistance in some of our other tests than the 
preceding. 
