'20 
The next series of experiments was designed to test the elfect of 
glycerin upon the organisms of pus. both in pure culture and the pus 
itself. 
For this purpose pus was obtained from various sources, abscess of 
hand, axill ary abscess, abscess of face, and a bone felon. The inflam- 
matory exudate was immediately planted in varying percentages of 
glycerin and examined from time to time. F or comparison the micro- 
cocci were plated out in pure culture and exposed to glycerin in like 
manner imder exactly the same conditions. 
PUS— STAPHVLOCOCCrS PYOGESPS AVREVS- 
Pn5 iDDiii an abscess of the hand conTaining Staphylococcus pyogenes aureus in pure 
culture. plante«i in varving percentages of glycerin (G) in liistiUed water and kept in 
the incubator at 37= C. Fr»Dm time to time small portions of the emulsion were <irawn 
off and plantei upon agar and tu bouillon. 
Day?. 
Pexcenrage of z. 
yc-erm. 
1 
Bemarks. 
10 
20 
30 
60 
TO 
Sl' 
SO 
lOij 
_ 
- 
- 
- 
- 
- 
- 
- 
- 
- 
AH marked — save no sTt-wth. 
in broth. 
U 
21 - 
■>; 

STAPHYtOCOCCrS rs' PUKE CULTUEE FEOil THE ABOVE PUS. 
A pure culture of Staphylococcus pyogenes aureus isolate*! from the above pus obtained 
from abscess of the hand was planted in various percentages of glycerin and tested as 
above. 
Day?- 
Percentage of g 
Lycerin. j 
! 
Eemark?, 
10 
20 
30 
4*j 
-50 
eo 
70 
SO 
90 1 
100 ' 
I 
1 
AH marked — gave no gnovrth 
in broth. 
11 
- 
- 
- 
_ 
m 
2S 

49 
