22 
Pus from an acute abscess of the face, containing Staphylococcus pyogenes aureus in 
pure culture, planted in varying percentages of glycerin (G) in distilled water and 
kept in the incubator at 37° C. From time to time small portions of the emulsion 
were drawn off and planted upon agar and in bouillon. 
Days. 
Percentage of glycerin. 
Remarks. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
7 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
1 ++++++ + 
All marked — gave no growth 
in broth. 
14 
21 
28 
35 
42 
49 
56 
STAPHYLOCOCCUS IN PUEE CULTURE FROM ABOVE PUS. 
A pure culture of Staphylococcus pyogenes aureus isolated from the above pus obtained 
from acute abscess of the face was planted in various percentages of glycerin and 
tested as above. 
Days. 
Percentage of glycerin. 
Remarks. 
10 
20 
30 
40 
50 
60 
70 
80 
90 
100 
7 
+ + + + 1 1 
+ 
+ 
+ 
+ 
+ 
+ + + + 1 I 
- 
- 
- 
- 
- 
- 
- 
All marked — gave no growth 
in broth. 
14 
21 
28 
35 
42 
