23 
Pus from a bone felon of the finger, containing Staphylococcus pyogenes alhus in pure 
culture, planted in various percentages of glycerin (G) in distilled water and kept 
in the incubator at 37° C. From time to time small portions of the emulsion were 
drawn off and planted upon agar and in bouillon. 
Days. 
Percentage of glycerin. 
Remarks. 
10 
20 
30 
40 
50 60 
70 
80 
90 
100 
7 
+ ++++I 
+ + + + 1 1 
+ 
+ 
+ 
+ 
- 
- 
- 
- 
- 
- 
- 
All marked — gave no growth 
in broth. 
14 
21 
28 
35 
42 
STAPHYLOCOCCUS IN PUEE CULTUKE FROM ABOVE PUS. 
A pure culture of Staphylococcus pyogenes alhus isolated from the above pus obtained 
from a bone felon of the finger was planted in various percentages of glycerin and 
tested as above. 
Days. 
Percentage of glycerin. 
Remarks. 
10 
20 30 
40 
50 
60 
70 
80 
90 
100 
7 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
1 1 1 ++++ + 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
- 
— 
— 
- 
— 
- 
— 
All marked — gave no growth 
in broth. 
14 
21 
28 
35 
42 
49 
56 
From these experiments it will be seen that glycerin has the power 
of destroying pus organisms whether in pure culture or in the pus 
itself within two weeks when exposed at these temperatures. As 
these tests were made in the incubator at 37° C. they can not be taken 
as an evidence of what glycerin may always do in vaccine virus when 
kept cool. 
TETANUS IN GLYCERIN. 
VIABILITY OP TETANUS IN GLYCERIN. 
On January 2, 1903, tetanus was planted into 1,000 c. c. of ordinary bouillon and 
grown two weeks in a Novy jar at 37° C. Examination of the growth showed that 
it was a pure culture. The spores and toxin were then separated by filtration and 
the toxin was set aside. ■ 
The spores were diluted with distilled water and filtered. The residue was again 
suspended in water and filtered several times iii order to remove the toxin. 1,000 
c. c. were used in the washing. 
