25 
Tetanus spores — Continued. 
AFTER THIRTY DAYS’ EXPOSURE TO GLYCERIN. 
[+ = typical symptoms of tetanus; — = no symptoms of tetanus; k - - killed; d = died.] 
Quantity inoculated into mice, per- 
centages of glycerin, and temper- 
ature. 
1st 2d 
day. day. 
Incubator, 37° C.- 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Ice chest, 10° to 12° C.: 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Room, about 20° C.: 
0.05 c. c. 10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Result. 
3d 
day 
4th 
day. 
5th 
day. 
6th 
day. 
7th 
day. 
8th 
day. 
9th 
day. 
10th 
day. 
11th 
day. 
12th 
day. 
+ k 
_ 
_ 

_ 

__ 
+k 
+k 
I""' 
j 
1 
+k 
+k 
AFTER SIXTY DAYS’ EXPOSURE TO GLYCERIN. 
Incubator 37° C. : 
0.05 c. c. distilled water 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
80 per cent glycerin 
100 per cent glycerin 
Ice chest, 10° to 12° C. : 
0.05 c. c. distilled water 
- 
+d 
+k 
+k 
+k 
+k 
- 
- 
- 
- 
- 
- 
- 
- 
- 
- 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
1 
j 
80 per cent glycerin 
1 
i 
100 per cent glycerin 
Room, about 20° C.: 
0.05 c. c. distilled wa.ter 
1 + + + + 1 1 
+ 
+k 
+k 
+k 
+k 
+k 
+ 
+ 
+ 
+ 
-1- 
+ 
+ 
+ 
20 per cent glycerin 
40 per cent glycerin 
60 per cent glycerin 
1 
80 per cent glycerin 

100 per cent glycerin 
AFTER NINETY DAYS’ EXPOSURE TO GLYCERIN. 
Incubator, 37° C.: 
0.05 c. c. distilled water 
10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Ice chest, 10° to 12° C.: 
0.05 c. c. distilled water 
10 per cent glycerin 
30 per cent glycerin 
50 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
Room, about 20° C.: 
0.05 c. c. distilled water 
10 per cent glycerin 
30 per cent glycerin 
60 per cent glycerin 
70 per cent glycerin 
90 per cent glycerin 
i 
- 
+ + + + 1 
+ 
+k 
+k 
-1- 
+ 
+ k 
+ k 
+ k 
- 
— 
? 
1 ++ 1 1 
1 1 ++ 1 
+ 1 
- 
- 
- 
- 
- 
- 
1 - 
h 
- 
