8 
Page. 
The toxine 35 
Preparation 35 
The culture 35 
The bouillon 35 
Neutralization 39 
Bottling and preserving 40 
Testing 42 
Determining the L-f- dose 42 
Determining the L® dose 43 
Seasoning the test poison 43 
The stage of equilibrium i 44 
Kesults of testing toxin No. 7 for L+ dose 44 
Tables for diluting toxines for MLD 45 
Tables for diluting toxines for L-|- dose 48 
The antitoxin 50 
Preparation 50 
Bottling and preserving 52 
Dissolving the dried serum 54 
Testing 54 
Methods used in making the unit 55 
The glassware 55 
“ Meniscus visir blende” of Doctor Gockel 58 
Cleaning the glassware 58 
The diluting fluid 58 
Bacterial precautions 58 
Method of using pipettes 59 
Capacity pipettes 60 
Delivery pipettes 63 
Method of inoculating the animals 65 
Description of syringe used 66 
The guinea pig 67 
Weight 68 
Technique of operation 68 
Effect upon the guinea pig 71 
Examination of serums made by licensed manufacturers 73 
Purchased on the open market 73 
Dilinquencies — how corrected 73 
Methods of testing; 
For potency 74 
. . For purity . , 74 
For preservatives 74 
Tables for diluting the serum 76 
Serum antidiphthericum in the Pharmacopoeia 79 
Official methods used in Germany for testing diphtheria toxine and antitoxin. . 81 
Bibliography - - 85 
