44 
n 
against the immunity unit every week or two in order to assure oui- 
selves that they are not undergoing change. A number of carefully 
tested toxines are alwa^^s kept on hand, so that we nm^ have a double 
test of the serums we are standardizing, as well as to guarantee against 
changes in the toxines which ma}" set in suddenly. 
The exact standardization of a toxine takes considerable time and 
we therefore can not depend upon one poison. The exact L+ dose 
of a well-seasoned toxine is the keynote of the whole testing opera- 
tion. Without this test dose good results are impossible. Every 
effort must be made to obtain a reliable poison that answers all the 
requirements and, having such a toxine, to maintain it under the most 
careful conditions in order to keep it in a state of equilibrium as long 
as possible. As the toxine grows old the tests naturally multiph^ in 
number and increase its reliability for the purpose of determining the 
unit strength of antitoxins. 
On account of the importance of the L+ dose I exhibit here a series 
of L+ tests showing the character of the results obtained by the 
poison after it has gained its stage of equilibrium. 
Table giving the results of tests on Toxine Xo. 7 for L— dose, us-ing 0.21 c. c. of the toxine 
against one immunitii unit, from October 24, 1904, to December 22, 1904- 
Guinea pig 
Xo. 
Time of 
death. 
Eemarks. 
1 Guinea pig 
Xo. 
Time of 
death. 
Remarks. 
Dys. ?ir$. 1 
Dys. hrs. 
395 1 
3 
0 1 
859 
2 
20 
Usual reactions. 
453 1 
3 
5 
860 
2 
15 
Do. 
4^ 
5 
15 
903 
3 
^ 
3 
9 
904 
3 
16 
545 
3 
21 
905 
6 
2 
546 
3 
5 1 
948 
3 
2 
547 
3 
18 ! 
949 
3 
2 
601 
5 
16 i 
950 
3 
18 
60'^ 
2 
17 
Clear serous fluid in pleu- 
1 951 
3 
18 
ral cavities. 
984 
3 
6 
603 ! 
3 
13 
985 
1 
18 
Pneumonia. 
604 
3 
6 
986 
1 
17 
Peritonitis; serous exu- 
605 
1 
18 
Omentum, mesentery, and 
date; omentum very red. 
broad ligament inject- , 
987 
5 
6 
ed, etc. 
988 
1 
17 
! Pneumonia. 
621 
3 
2 : 
989 
2 
6 
Clear serum in pleural 
622 
2 
19 
Clear fluid in pleural cav- 
cavities. 
ity. 
1063 
4 
20 
623. 
3 
2 
1064 
3 
0 
624 
3 
14 
1065 
3 
12 
625 
3 
1066 
4 
13 
693 
3 
18 
1067 
3 
8 
694 
4 
11 
1068 
3 
4 
695 
3 
14 
1069 
1 
17 
Pneumonia, both upper 
696 
4 
8 
lobes. 
697 
3 
16 
1 1124 
2 
17 
Spleen and liver show 
750 
3 
19 
sharply defined caseous 
751 
^ 2 
12 
Peritoneum discolored 
areas. 
and petechia? through- 
1125 
1 4 
16 
out. 
1126 
3 
17 
752 
' 2 
12 
Some peritoneal reaction 
1127 
5 
17 
and clear serum in pleu- 
1128 
2 
17 
Usual reaction. 
rae. 
1129 
3 
6 
753 
1 9 
14 
Nothing unusual, adre- 
1150 
3 
8 
1 nals congested, local re- 
i 1151 
1 
19 
Severe peritoneal reac- 
i action. 
tion. 
754 
1 2 
17 
Do. 
1152 
9 
17 
Peritoneal fat injected. 
808 
i 3 
17 
i 1153 
I 3 
11 
809. . 
4 
14 
1154 
2 
16 
Clear serum in pleural 
855 
2 
20 
Usual reaction. 
cavities. 
856 
: 4 
4 
1155 
2 
12 
Usual reaction. 
857 
3 
9 
I 1156 
3 
11 
858 
4 
4 
