45 
During two months covered by the table every one of the 68 
animals injected with 0.21 c. c. of this toxine, mixed with 1 immunit}^ 
unit, died acutely. None recovered. Most of the guinea pigs died on 
the fourth day. As we usually inoculate our animals at about 3 o’clock 
in the afternoon, animals dying between three days two hours and 
four days two hours will appear on the autops}^ table on the fourth 
day. 
Animals dying before the fourth day usually show at autopsy some 
reason for this increase of susceptibility. 
The following tables used for diluting our toxines for MLD and L+ 
doses will be found useful : 
TABLES FOR DILUTING TOXINES FOR NLD. 
First dilution. 
1+99 = 1:100 
Second dilution. 
1 of first dilution+49 = l: 5000 
1 of first dilution+39=l: 4000 
1 of first dilution+29 = l: 3000 
1 c. c.=.0002 
1.5 c. c.=.0003 
2 c. c. = .0004 
2.5 c. c. = .0005 
3 c. c. = .0006 
0.8 c. c.=.0002 
1 c. c. =.00025 
1.2 c. c.=.0003 
1.6 c. c.=.0004 
2 c. c. = .0005 
2.4 c. c.=.0006 
2.8 c. c. = .0007 
3.2 c. c. = .0008 
0. 9 c. c. = .0003 
1 c. c. =.00033 
1.2 c. c. =.0004 
1.5 c. c. = .0005 
1.8 c. c. = .0006 
2.1 c. c.=.0007 
2.4 c. c.=.0008 
2.7 c. c. = .0009 
3 c. c. = .001 
1. c. c. = .001 
1.5 c. c. = .0015 
2 c. c. = .002 
2.5 c. c. = .0025 
3 c. c. = .003 
1 of first dilution+9 = l:1000 
