65 
METHOD OF IXOCULATIXG THE AXIMALS. 
The method used for inoculating’ the ani- 
mals has been described in Bulletin No. 
19 of the H 3 ^g’ienic Laboratory, Public 
Health and Marine-Hospital Service, en- 
titled ‘*A method for inoculating animals 
with precise amounts.” and onH the essen- 
tial features as applicable to this work will 
be repeated. 
The syringe used for carrying out this 
technique is a modification of the old Koch 
stu’inge. 
The needles are sterilized separately by 
the usual method of boiling in a 1 per cent 
sodium carbonate solution. 
The needle is then screwed on the barrel 
of the syringe and the joint tested b}" 
drawing some sterile salt solution in and 
out several times. If the joint is tight and 
the needle pervious, the outside is dried 
with a little piece of sterile gauze and the 
needle is now plunged into a jar of steril- 
ized albolene. The albolene acts as a tem- 
porary plug, preventing any of the fluid 
that is placed in the s}H’inge from escaping 
until it is injected into the animal. 
The necessaiT number of svringes are 
prepared, one for each animal. In testing 
diphtheria toxine and antitoxin we some- 
times have a battery of fift\" or sixtv thus 
prepared, arranged on the rack as shown 
in the accompanying diagram (fig. 15). 
The amount of fluid desired to inject 
into the animal is now carefully measured 
di recti V into the barrel of the syringe. 
This is injected into the animal and then, 
without withdrawing the needle, the bulb 
is removed. The necessary quantit\^ of 
salt solution is quicklv run into the bar- 
I’el, washing down the sides, and this in 
turn is injected into the animal. 
When the s^u’inges contain fluid they 
can not be inverted, as is the case with a 
piston syringe. It is not necessaiT, how- 
-No. 21-05- 
o 
