I 
35 
; human case. These cows became ill, had a rise in temperature, and 
; on the fifth day there appeared upon the udder an eruption character- 
I ized by papules, vesicles, and crusts. He states that he isolated the B. 
^phtheriae from the vesicles, pustules, and milk. Other experiment- 
’s ® ^ have however failed to get similar results. The Klebs-Loffler 
oacillus has been isolated from market milk by Bowhill,"^ Eyre,"^ 
Klein,® and Dean and Todd.^ 
KLEBS-LOFFLER BACILLI IN MILK. 
;t ■ 
r , Dean and Todd reported that in certain families supplied with 
i; ilk from two cows there occurred 2 cases of clinically typical diph- 
\l theria and 3 of sore throat, that in one family using the milk only 
( after sterilization no case occurred. Inspection of the cows showed 
i' papules, crusts, and ulcers on the teats and udders. One of the cows 
' seemed well and gave apparently normal milk; the other had a 
I mammitis and gave a scanty, ropy, semipurulent and slightly blood- 
' , tinged milk. Cultures were made from the throat of one of the diph- 
theria patients and also from the ulcers and milk of each cow, and 
typical Klebs-Loffler bacilli were isolated in all cases. The milk of 
" cow with mammitis also contained streptococci. The bacillus 
ited was virulent and markedly pathogenic to guinea pigs, but 
aiphtheria antitoxin protected guinea pigs against large doses. The 
udder eruption was shown to be contagious to cows and capable of 
spread by the hands of the milker, but no B. diphtherise were found 
in vesicles and ulcers of the secondary bovine cases. Calves were not 
protected from this disease by diphtheria antitoxin, nor by this dis- 
ease from cowpox. The conclusions drawn were that the ulcers on 
the udders had become secondarily infected with B. diphtherise, 
probably accidentally from some apparently healthy throat, and that 
udder affection was a separate disease. 
Eyre ^ has shown the ability of the B. diphtherise to proliferate in 
raw milk drawn from the cow under aseptic conditions as follows : 
0 hours. 
24 hours. 
1 48 hours. 
7 days. 
B. rtiphthprifp 
39 
1,170 
22,000 
19,000,000 
« Abbott (A. C.), Jour. Path. & Pact., 1894, II, p. 35. 
^ Ritter, Centralblatt f. Bakt., Referat, 1896, XIX, p. 662. 
® Bowhill, Veterinary Record, 1899, April 8th. 
^ Eyre, Brit., Med., Jour., 1899., II, p. 586. 
® Klein, Journal Hygiene, Camb., 1901, I, p. 85. 
f Dean & Todd, Jour. Hygiene, Camb., 1902, II, p. 194. 
£Eyre, loco citato. 
