174 
Delepine, S. Brit. Med. Jour., 1898, vol. 2, p. 918. 
In a popular lecture, gives the following results with milks col- 
lected by health officers of Liverpool, Manchester, and elsewhere : 
(a) Seven specimens unmixed milk from cows showing no evidence of tuber- 
culosis. Tubercle bacilli in none of the specimens. 
(&) Twenty-two specimens unmixed milk from cows showing distinct evi- 
dence of tuberculosis and in 6 cases udder involvement. Tubercle bacilli in 
27.24 per cent. 
(c) Fifty-four specimens mixed town milk. Tubercle bacilli in 5.55 per 
cent. 
(d) One hundred and twenty -five specimens country farm milk. Tubercle 
bacilli in 17.6 per cent. 
The presence of tubercle bacilli was determined by inoculation of 
guinea pigs and their post-mortem examination. 
Petri. Zum Nachweis der Tuberkelbacilli in Butter und Milch. Arb. a. d. 
kais. Ges.-Amt., XIV, 1898, p. 1. 
Milk specimens taken from various places in Berlin. Centrifuged 
in 150 cubic centimeter flasks. Three cubic centimeters each of 
cream, skim milk, and sediment injected into 4 guinea pigs (12 
animals for each specimen). Later, on account of the lack of 
animals, 5 cubic centimeters from each specimen were inoculated into 
each of 4 guinea pigs. 
Sixty-four specimens were examined. Tubercle bacilli were dem- 
onstrated in nine (14 per cent). Tubercle bacilli-like rods, not true 
tubercle bacilli in 4 specimens (6.3 per cent). 
It appears that 200 out of the 478 animals died, mostly of peri- 
tonitis within the first three weeks, thus eliminating 7 specimens 
from consideration, and leaving 57 on which to base a percentage of 
incidence. As 9 of these contained tubercle bacilli, the corrected 
percentage would be 17.5. 
The importance of using a large number of animals for each speci- 
men is shown by the fact that in only 3 of the 9 positive specimens 
did more than 1 animal become tuberculous. In these 3 cases there 
were 2. 
Ascher. Untersuchungungen von Butter und Milch auf Tuberkelbacillen. Zeit. 
f. Hyg., Bd. 32, 1899, S. 329. 
Injected 17 specimens of Koningsberg milk into guinea pigs intra- 
peritoneally. One of the animals became tuberculous. The milk 
was partly centrifuged, and the cream and sediment injected, and 
partly uncentrifuged. No other acid-fast bacilli found. 
The first streams from the milking were used, which may account 
for the lower percentage of infected specimens detected by him than 
by Rabinowitsch, who used the last part of the milking. The com- 
