471 
Table No. 10 . — Milk from healthy cow (No. 2) inoculated one and one-half hours 
after milking. 
L Controls, same milk heated in Arnold sterilizer to 100° C. for ten minutes. Organisms 
used were from 24-hour agar cultures.] 
1 
1 
Bacteria per 
loop at once. 
Bacteria per 
loop after 8 
hours at 37° 
C., tube 
moderately 
shaken. 
SB, lactis aerogenes in raw milk 
1,200 
1,200 
3, 440 
2, 160 
2,120 
2, 660 
4 
4,400 
( a ) 
4,107 
( a ) 
1,220 
30, 000 
6 
Ib. lactis aerogenes, control 
■ B. typhosus in raw mi Ik 
fl B t 53 >hosus, control 
Staphylococcus pyogenes aureus in raw milk 
Staphylococcus pyogenes aureus, control 
Original milk 
Bacteria per 
loop after 8 
hours at 37° 
C., culture 
vigorously 
agitated 5 
minutes and 
mixed with 
a pipette. 
14,000 
7,560 
2, 070 
“ Innumerable. 
The next experiment was designed to show whether the phenome- 
non of germicidal action could not be duplicated by the addition of 
typhoid agglutinating serum to milk that had been boiled. 
Table No. 11 . — Milk from healthy cow {l^o. 2) planted one and one-half hours 
after milking. 
[Controls, same milk boiled— 24-hours old agar culture used.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 2^ 
hours at 
37° C. 
Bacteria 
per loop 
after 4^ 
hours at 
37° C. 
Bacteria 
per loop 
after 6i 
hours at 
37° C. 
Bacteria per loop after 
8i hours at 37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
B. typhosus in raw milk 
1,880 
1,380 
1,060 
1,480 
1,980 
12,200 
B. typhosus in same milk 
boiled (control) 
2,120 
4,020 
a b 800, 000 
, (“) 
( a ) 
( a ) 
B. typhosus in raw milk plus 
1 drop typhoid serum 
2, 100 
2,040 
1,920 
2,360 
1,260 
a b 20, 000 
B. typhosus in boiled milk 
plus 1 drop typhoid serum. 
2,280 
2, 360 
7,020 
6,480 
10, 860 
a b 60,000 
B. typhosus in bouillon plus 
1 drop typhoid serum. 
1,830 
970 
2,920 
9, 180 
11,160 
a b 100, 000 
Original milk 
0 
1 
2 
2 
2 
46 
“ Innumerable. 
»About. 
The tj^phoid serum used in this experiment was a strongly agglu- 
jtinating horse serum (strength over 1 : 10,000). 
j It will be seen that boiled milk plus typhoid agglutinin acts much 
the same as the raw milk. The contrast in each case with the con- 
trol (boiled milk) is striking. 
