472 
The fact that agglutination plays a prominent role in this phe- 
nomenon is well illustrated in this table by the fact that the bacterial 
clumps may be shaken apart. This was confirmed by the micro- 
scopic examinations of stained smears in each case. 
Table Xo. 12. — l[Uk from healthy cow (Xo. 2). 
[Plantings one hour after milking.] 
Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria 
per loop 
after 
5 hours at 
1 37° C. 
I 
Bacteria per loop after 
7 hours at 37° C. 
Moder- Mixed 
ately with 
shaken. pipette. 
i 
B. typhosus in raw milk 
B. typhosus in boiled milk 
48-bour B. coli culture in raw milk 
Original milk 
5.400 
6.400 
16,000 
2 
4,680 
5,040 
12,000 
1 
4, 720 
a 6 100,000 
11,000 
2,250 ' 
a 6 200, 000 ' 
9,000 
5,400 
a 6 200, 000 
a 6 40, 000 
112 
“ Innumerable. '’About. 
Table Xo. 13 . — Milk from healthy cow (Xo. 2). 
[Plantings one hour after milking.] 
1 
; Bacteria 
per loop 
at once 
after in- 
oculation. 
Bacteria 
per loop 
after 
3 hours at 
37° C. 
Bacteria 
per loop 
after 
5 hours at 
37° C. 
Bacteria per loop after 
7 hours at 37° C. 
Moder- 
ately 
shaken. 
Mixed 
with 
pipette. 
1 loop faeces and hay emulsion in raw milk . 
1 
720 
1 
660 
480 
420 
4, 320 
1 drop faeces and hay emulsion in raw milk . 
4,860 
1 ’ 
4,640 
6,000 
3,780 
9, 720 
5 drops faeces and hay emulsion in raw 
1 
milk 
10,500 
1 
7,500 
9,900 
13,000 
20,000 
Table Xo. 14. — Milk from healthy cow (Xo. 2) planted one and one-half hours 
after milking. 
[Controls same milk boiled.] 
Bacteria 
per loop 
at once 
' Bacteria 
1 per loop 
after 2i 
hours at 
37° C. 
1 Bacteria 
per loop 
after 4^ 
hours at 
37° C. 
1 
Bacteria 
per loop 
after 6i 
hours at 
37° C. 
Bacteria per loop 
after hours at 
37° C. 
after in- 
ocula- 
tion. 
Moder- 
ately 
shaken. 
! Mixed 
1 with 
pipette. 
1 drop cow faeces and hay suspension in 
raw milk 
1 drop cow faeces and hay suspension in 1 
0 ! 
1 
0 I 
f ! 
3 
26 
1 1 
480 
boiled milk (control) 
11 1 
43 , 
18 
80 
1,260 i 
4 drops cow faeces and hay suspension in j 
raw milk 
51 
2 ! 
124 
450 
1,800 
5,400 
4 drops cow faeces and hay suspension in 
boiled milk (control) 1 
7 
28 
720 
3,300 
Original milk ! 
"1 
2 
2 
2 1 
46 
