476 
Table No. 17 . — Milk from healthy cow {'No. 2). 
[Inoculated one and one-half hours after milking. Controls, same milk heated to boiling. 
Organisms from 24-hour agar culture, using suspension in water of condensation in test 
tube.] 
Bacteria 
per loop at 
once after 
inocula- 
tion. 
Bacteria per loop after 
10 hours at 37° C. 
Test tube 
mod- 
erately 
shaken. 
Vigorous 
agitation 
of culture 
for 5 min- 
utes. 
B. typhosus in raw milk 
1,760 
2,100 
2,740 
2,300 
2,460 
3,200 
1, 420 
1,830 
1,700 
(«) 
(a) 
(«) 
(«) 
(«) 
900 
6, 640 
42 
12, 500 
B. typhosus in control 
B. para typhosus A in raw milk 
(«) 
B. para typhosus A in control 
B. para typhosus B in raw milk 
(«) 
B. para typhosus B in control 
Staph, pyog. aureus in raw milk 
9, 100 
Staph, pyog. aureus in control 
Original milk 
“ Innumerable. 
THE EFFECT OF DILUTION. 
AVhole raw milk from a healthy cow (No. 2) was diluted in the 
proportion of 1 volume of milk to 3 volumes of distilled water. 
Inoculations were made two hours after milking; 24-hour agar cul- 
tures used. 
Table No. 18. 
Bacteria 
per loop at 
once after 
inocula- 
tion. 
Bacteria per loop after 
10 hours at 37° C. 
Tube 
shaken 
moder- 
ately. 
Culture 
yigorously 
agitated 6 
minutes 
and mixed 
with a 
pipette. 
B. typhosus in diluted milk (l-f3) 
11,000 
35.000 
16. 000 
3,000 
5,400 
36, 000 
3.000 
1,200 
a b 160, 000 
4.000 
33 
a & 360, 000 
25. 000 
15.000 
B. typhosus in same milk undiluted 
B. typhosus in sterilized milk (control) 
B. lactis aerogenes in diluted milk (1-1-3) 
67, 000 
92 
B. lactis aerogenes in same milk undiluted 
B. la, cti.s aerogenes in sterilized milk (control) 
a Innumerable. 
i> About. 
