592 
bacteriological investigation had already been made and reported 
upon by Mr. Kellermann, Bureau of Plant Industry, of the dairies in 
the District of Columbia and in the countr^^ immediately contiguous 
thereto, and it was not deemed necessary to duplicate this work. 
The method of procedure in the present investigation consisted in 
visiting from 4 to 8 or more dairies a day, and while the inspection of 
the dairies and barns was in progress, cultures were prepared from 
samples of the water, and the source and surroundings of the water 
supplies were recorded in each case upon a specially prepared blank 
form. Below is given a specimen of one of these forms which was 
actually employed. 
In these blanks the parts applicable in each case were underscored, 
and the names, dates, and remarks filled in. In the accompaning 
blank the words which were underscored in this case are shown, and 
the parts filled in are shovm in italics. 
Name, Buckingham Industrial School. Date, 6-10-07. 
Address, Buckeystown, Md. 
Covered open spring not protected from surface drainage by tight coping. Con- 
• ducted through pipe by ram gra\ity. 
Well bored. Open with bucket and windlass. Pump suction chain. Depth, 109 
feet. Through impervious layer rock. 
Cistern. Slate metal shingle roof. No means of turning off first water. Brick 
partition. 
Situation good fah bad. Ground slopes away toward level. Ground not subject 
to pollution with human or animal excrement. 
Remarks: Well situated and well protected in all respects. Pump inside inclosed 
base of windmill tower. Door kept locked. Water conducted through pipe to dairy 
house about 100 yards away. All sources of pollution remote. 
[Front face filled out at the source of supply.] 
Number of colonies per 1 c. c. : 
At 35° C. for 24 hours, 
At room temperature for 48 hours, 210. 
PRESUMPTIVE TEST FOR B. COLi: 
Number of acid-producing colonies on litmus agar per 1 c. c., 63. 
Neutral — red agar: 
Fermentation in one-tenth c. c., — In 1 c. c.,-f 
Color in one-tenth c. c., — In 1 c. c.,-|- 
Milk coagulated, reaction unchanged. Indol reaction present. Diagnosis para- 
colon. 
[Reverse face filled out at the laboratory.] 
In addition to the cultures, a half-gallon glass-stoppered bottle 
carefully cleaned in the laboratory was filled ^\dth the water under 
inspection, and shipped to Washington for chemical examination. 
The media employed for obtaining the cultures consisted in all 
cases of litmus-lactose agar, and neutral-red-lactose agar. These. 
